龈沟液中乳酸脱氢酶活性作为正畸牙齿移动的一项标志物
Lactate dehydrogenase activity in gingival crevicular fluid as a marker in orthodontic tooth movement.
作者信息
Alfaqeeh Sarah A, Anil Sukumaran
机构信息
Department of Pediatric Dentistry and Orthodontics, College of Dentistry, King Saud University, Post Box: 60169, Riyadh11545, Kingdom of Saudi Arabia.
出版信息
Open Dent J. 2011;5:105-9. doi: 10.2174/1874210601105010105. Epub 2011 Jul 7.
OBJECTIVES
This study aims at analyzing the changes in gingival crevicular fluid (GCF) lactate dehydrogenase (LDH) activity during orthodontic movement.
METHODS
Twenty patients all requiring first premolar extractions were selected and treated with conventional straight wire mechanotherapy. Canine retraction was done using 125 g Nitinol closed coil springs. The maxillary canine on one side served as the experimental site while the contralateral canine served as the control. GCF was collected from the canines before initiation of retraction, then 1 hour after initiating canine retraction, followed by 1 day, 7 days, 14 days and 21 days. GCF LDH levels were estimated and compared with the control site.
RESULTS
The results revealed significantly higher LDH levels on the 7(th), 14(th) and 21(st) day at the sites where orthodontic force had been applied. The levels also showed a significant increase from 0 hour to the 21st day. Peak levels were seen on 14(th) and 21(st) day following initiation of retraction.
CONCLUSIONS
The study showed that LDH could be successfully estimated in the GCF and its increased levels could indicate active tooth movement, which could aid the clinician in monitoring active orthodontic tooth movement.
目的
本研究旨在分析正畸移动过程中龈沟液（GCF）乳酸脱氢酶（LDH）活性的变化。
方法
选取20例均需拔除第一前磨牙的患者，采用传统直丝弓矫治技术进行治疗。使用125g镍钛闭合曲弹簧进行尖牙后移。一侧上颌尖牙作为实验部位，对侧尖牙作为对照。在开始后移前从尖牙采集龈沟液，然后在开始尖牙后移1小时后采集，随后在1天、7天、14天和21天采集。对龈沟液LDH水平进行评估并与对照部位进行比较。
结果
结果显示，在施加正畸力的部位，第7天、14天和21天的LDH水平显著更高。从0小时到第21天，这些水平也显著升高。后移开始后第14天和21天出现峰值水平。
结论
该研究表明，可成功测定龈沟液中的LDH，其水平升高可能表明牙齿在进行活跃移动，这有助于临床医生监测正畸牙齿的活跃移动情况。
Zotero 插件