Slomianny C, Prensier G, Charet P
J Protozool. 1985 Feb;32(1):1-5. doi: 10.1111/j.1550-7408.1985.tb03003.x.
By serial sectioning and 3D reconstruction we have been able to demonstrate that the type of system for hemoglobin digestion in two strains of Plasmodium berghei, N and RC, is dependent on the maturity of the host cell. In parasites growing in erythrocytes, both systems for the endocytosis of hemoglobin-micropinocytosis and the cytostomal system (i.e. a cytostome budding a cytostomal tube that releases food vacuoles)-are fully functional and produce a great quantity of residual pigment. Parasites growing in reticulocytes have a disrupted cytostomal system; no tube is formed and only food vacuoles are visible in their cytoplasm. Residual pigment is smaller in size and in quantity. The reduced quantity of pigment in reticulocytes is explained by our observation of the exocytosis of pigment. We propose a hypothesis that relates the process of degradation of hemoglobin to the maturity of the host cell and a possible mechanism of protection against chloroquine, a drug known for its affinity for malarial pigment.
通过连续切片和三维重建，我们已经能够证明，伯氏疟原虫N株和RC株中血红蛋白消化系统的类型取决于宿主细胞的成熟度。在红细胞内生长的寄生虫中，血红蛋白内吞的两种系统——微胞饮作用和胞口系统（即胞口长出释放食物泡的胞口管）——都完全起作用，并产生大量残余色素。在网织红细胞内生长的寄生虫的胞口系统遭到破坏；没有形成管子，其细胞质中仅可见食物泡。残余色素在大小和数量上都较少。网织红细胞中色素数量减少是由于我们观察到色素的胞吐作用。我们提出一个假说，将血红蛋白的降解过程与宿主细胞的成熟度联系起来，并提出一种可能的针对氯喹的保护机制，氯喹是一种以对疟色素有亲和力而闻名的药物。
