University of Arkansas at Little Rock, Little Rock, AR, USA.
Methods Mol Biol. 2024;2832:171-182. doi: 10.1007/978-1-0716-3973-3_12.
Stress can affect different groups of plant metabolites and multiple signaling pathways. Untargeted metabolomics enables the collection of whole-spectrum data for the entire metabolite content present in plant tissues at that point in time. We present a thorough approach for large-scale, untargeted metabolomics of plant tissues using reverse-phase liquid chromatography connected to high-resolution mass spectrometry (LC-MS) of dilute methanolic extract. MZmine is a specialized computer software that automates the alignment and baseline modification of all derived mass peaks across all samples, resulting in precise information on the relative abundance of hundreds of metabolites reflected by thousands of mass signals. Further processing with statistic and bioinformatic techniques will provide a comprehensive perspective of the variations and connections among groups of samples.
压力会影响不同植物代谢物群体和多种信号通路。非靶向代谢组学能够采集到植物组织中特定时间点存在的整个代谢物谱数据。我们提出了一种使用反相液相色谱连接稀甲醇提取物的高分辨质谱（LC-MS）对植物组织进行大规模非靶向代谢组学分析的彻底方法。MZmine 是一种专门的计算机软件，可自动对齐和修改所有样本中所有衍生质谱峰的基线，从而精确地反映出数千个质谱信号中数百种代谢物的相对丰度信息。通过统计和生物信息学技术的进一步处理，将提供关于样品组之间变化和联系的全面视角。
