Müller R, Curran T, Müller D, Guilbert L
Nature. 1985;314(6011):546-8. doi: 10.1038/314546a0.
Previous studies have suggested a role for c-fos in cellular differentiation in fetal membranes, haematopoietic cells and teratocarcinoma stem cells. In other cell types, such as fibroblasts, c-fos expression is normally very low, but is rapidly induced by peptide growth factors, implicating c-fos in growth control mechanisms. Here, we show that the TPA (12-O-tetradecanoylphorbol-13-acetate)-induced macrophage-like differentiation of HL60 human promyelocytic precursor cells is accompanied by the induction of both c-fos mRNA and protein within 15 min after treatment, suggesting a functional role for c-fos in this differentiation system. In quiescent terminally differentiated macrophages, expression of c-fos is inducible by the macrophage-specific growth factor colony-stimulating factor-1 (CSF-1). The kinetics of c-fos induction, however, are entirely different from those in growth factor-stimulated fibroblasts, supporting the view that the c-fos gene product may serve different functions in different cell types.
先前的研究表明，c-fos在胎膜、造血细胞和畸胎癌干细胞的细胞分化中发挥作用。在其他细胞类型中，如成纤维细胞，c-fos的表达通常非常低，但会被肽生长因子迅速诱导，这表明c-fos参与生长控制机制。在此，我们发现，TPA（12-O-十四酰佛波醇-13-乙酸酯）诱导HL60人早幼粒细胞前体细胞向巨噬细胞样分化，在处理后15分钟内伴随着c-fos mRNA和蛋白质的诱导，这表明c-fos在该分化系统中具有功能作用。在静止的终末分化巨噬细胞中，c-fos的表达可被巨噬细胞特异性生长因子集落刺激因子-1（CSF-1）诱导。然而，c-fos诱导的动力学与生长因子刺激的成纤维细胞完全不同，这支持了c-fos基因产物在不同细胞类型中可能发挥不同功能的观点。
