Birek C, Lui E, Dardick I
Department of Oral Pathology, Faculty of Dentistry, University of Toronto, Ontario, Canada.
Am J Pathol. 1993 Mar;142(3):917-23.
Tissue from 35 salivary gland tumors and 14 normal salivary glands was analyzed by in situ hybridization and computer-assisted morphometry for the expression of the c-fos oncogene. The normal salivary gland tissues were found to express c-fos focally, mainly in the acinar secretory cells. The majority of the cells in the normal tissues showed a high level of expression (47.74 +/- 5.31% of cells had 46 to 60 grains per cell and another 45.79 +/- 2.18% showed > 60 grains per cell). All the tumors examined exhibited a relatively low, uniform distribution of c-fos expression. For example, in the poorly differentiated adenocarcinomas, 96.83 +/- 04% of the cells were found to have < 15 grains per cell. A general linear model for multivariate analysis showed a significant difference between the various tumor types and the normal salivary gland tissues (P = 0.0001). These data support the hypothesis that salivary gland tumors belong to a group of epithelial neoplasias in which the loss of cellular differentiation is linked with underexpression of the c-fos oncogene.
通过原位杂交和计算机辅助形态测量法，对35例涎腺肿瘤组织和14例正常涎腺组织进行分析，以检测c-fos癌基因的表达。结果发现，正常涎腺组织中c-fos呈局灶性表达，主要见于腺泡分泌细胞。正常组织中的大多数细胞呈现高水平表达（47.74±5.31%的细胞每细胞有46至60个银粒，另有45.79±2.18%的细胞每细胞银粒>60个）。所有检测的肿瘤均表现出c-fos表达相对较低且分布均匀。例如，在低分化腺癌中，96.83±04%的细胞每细胞银粒<15个。多变量分析的一般线性模型显示，不同肿瘤类型与正常涎腺组织之间存在显著差异（P = 0.0001）。这些数据支持以下假说：涎腺肿瘤属于一组上皮性肿瘤，其中细胞分化的丧失与c-fos癌基因的低表达有关。
