Li Shang, Wang Yutong, Xu Geweirong, Xu Yuqing, Fu Cuiyan, Zhao Quanlin, Xu Linjie, Jia Xinzhou, Zhang Yumeng, Liu Yi, Qiao Jiaju
Department of Biotechnology, School of Life Sciences, Xuzhou Medical University, Xuzhou, China.
Department of Biophysics, School of Life Sciences, Xuzhou Medical University, Xuzhou, Jiangsu, China.
Front Microbiol. 2024 May 30;15:1341316. doi: 10.3389/fmicb.2024.1341316. eCollection 2024.
Microorganisms in biofilms are particularly difficult to control because of their increased survival and antibiotic resistance. Allicin and domiphen were employed to inhibit the microbial growth and biofilm formation of , , and strains.
Broth microdilution method and checkerboard assay were conducted to determine the efficacy of allicin combined with domiphen against , , and . Microbial biofilm formation was measured using the crystal violet staining method and fluorescence microscopy. And the total viable count of the biofilm cells on material surface after the treatment with antimicrobial reagents was calculated with the plate count technique.
The two drugs showed synergistic effects against the pathogens with a fractional bactericidal concentration of less than 0.38. The combination of 64 μg/mL allicin with 1 μg/mL domiphen dispersed over 50% of the biofilm mass of , , and . In addition, the drug combination reduced the total viable counts of and biofilm cells on stainless steel and polyethylene surfaces by more than 10 CFU/mL.
The combination of allicin and domiphen is an effective strategy for efficiently decreasing biofilms formation on various industrial materials surfaces.
生物膜中的微生物由于其更强的生存能力和抗生素耐药性而特别难以控制。大蒜素和度米芬被用于抑制 、 和 菌株的微生物生长及生物膜形成。
采用肉汤微量稀释法和棋盘法测定大蒜素与度米芬联合使用对 、 和 的疗效。使用结晶紫染色法和荧光显微镜观察微生物生物膜的形成。并用平板计数技术计算抗菌试剂处理后材料表面生物膜细胞的总活菌数。
两种药物对病原体显示出协同作用，部分杀菌浓度小于0.38。64μg/mL大蒜素与1μg/mL度米芬的组合分散了 、 和 超过50%的生物膜量。此外，该药物组合使不锈钢和聚乙烯表面的 和 生物膜细胞的总活菌数减少了超过10 CFU/mL。
大蒜素和度米芬的组合是一种有效策略，可有效减少各种工业材料表面的生物膜形成。
