Inhibitory effects of vorinostat (SAHA) against food-borne pathogen serotype Kentucky mixed culture biofilm with virulence and quorum-sensing relative expression.

is a food-borne microorganism that is also a zoonotic bacterial hazard in the food sector. This study determined how well a mixed culture of Kentucky formed biofilms on plastic (PLA), silicon rubber (SR), rubber gloves (RG), chicken skin and eggshell surfaces. interactions between the histone deacetylase inhibitor-vorinostat (SAHA)-and serotype Kentucky were examined utilizing biofilms. The minimum inhibitory concentration (MIC) of SAHA was 120 µg mL. The addition of sub-MIC (60 µg mL) of SAHA decreased biofilm formation for 24 h on PLA, SR, RG, Chicken skin, and eggshell by 3.98, 3.84, 4.11, 2.86 and 3.01 log ( < 0.05), respectively. In addition, the initial rate of bacterial biofilm formation was higher on chicken skin than on other surfaces, but the inhibitory effect was reduced. Consistent with this conclusion, virulence genes expression ( and ) and quorum-sensing (QS) gene () was considerably downregulated at sub-MIC of SAHA. SAHA has potential as an anti-biofilm agent against enterica serotype Kentucky biofilm, mostly by inhibiting virulence and quorum-sensing gene expression, proving the histone deacetylase inhibitor could be used to control food-borne biofilms in the food industry.