Bi Ran, Zhang Dan, Quan Rui, Lin Xiaoxian, Zhang Wen, Li Chuangang, Yuan Man, Fang Bing, Wang Dongliang, Li Yixuan
Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Health China Agricultural University Beijing China.
Hebei Edible Bird's Nest Fresh Stew Technology Innovation Center Langfang China.
Food Sci Nutr. 2024 Apr 1;12(6):4196-4210. doi: 10.1002/fsn3.4080. eCollection 2024 Jun.
Exposure to cigarette smoke directly damages the lungs and causes lung inflammation. The anti-inflammatory properties of edible bird's nest (EBN) have been reported. We aimed to determine the effect of EBN on pneumonia in a mouse model exposed to cigarette smoke. Fifty BALB/c mice were randomly divided into control, model, positive drug, low-dose EBN, and high-dose EBN groups ( = 10 each). Except for the control group, the mice in each group were exposed to four cigarettes once a day for 8 days. In addition, we validated the effects of EBN on A549 cells and investigated the mechanism by which EBN alleviates lung inflammation. Edible bird's nest (EBN) could alleviate the structural damage of lung tissue and the smoke-induced inflammatory response in mice. The best effect was observed at the high dose of EBN (0.019 g). The mice treated with EBN had a stronger ability than those in the model group to resist cigarette smoke stimulation, as indicated by a decrease in serum and lung inflammatory markers (interleukin 6 [IL-6], tumor necrosis factor-α [TNF-α], and interleukin 8 [IL-8]), an increase in serum interleukin 10 (IL-10) levels, and a decrease in the expression of inflammasome NOD-like receptor pyrin 3 (NLRP3). In addition, our cell experiments showed that EBN attenuated cigarette smoke-induced pulmonary inflammation mainly by inhibiting the tumor necrosis factor receptor 1 (TNFR1)/nuclear factor-kappa B (NF-κB)/NLRP3 pathway. These findings provide theoretical evidence for the positive nutritional qualities of EBN for the lung by demonstrating that it inhibits the TNFR1/NF-κB/NLRP3 signaling pathway, which prevents the development of cigarette smoke-induced pulmonary inflammation.
接触香烟烟雾会直接损害肺部并引发肺部炎症。已有报道称食用燕窝（EBN）具有抗炎特性。我们旨在确定EBN对暴露于香烟烟雾的小鼠模型中肺炎的影响。将50只BALB/c小鼠随机分为对照组、模型组、阳性药物组、低剂量EBN组和高剂量EBN组（每组n = 10）。除对照组外，每组小鼠每天暴露于4支香烟，持续8天。此外，我们验证了EBN对A549细胞的作用，并研究了EBN减轻肺部炎症的机制。食用燕窝（EBN）可减轻小鼠肺组织的结构损伤和烟雾诱导的炎症反应。在高剂量EBN（0.019 g）时观察到最佳效果。与模型组相比，接受EBN治疗的小鼠抵抗香烟烟雾刺激的能力更强，表现为血清和肺部炎症标志物（白细胞介素6 [IL-6]、肿瘤坏死因子-α [TNF-α]和白细胞介素8 [IL-8]）减少，血清白细胞介素10（IL-10）水平升高，炎性小体NOD样受体吡啉3（NLRP3）表达降低。此外，我们的细胞实验表明，EBN主要通过抑制肿瘤坏死因子受体1（TNFR1）/核因子-κB（NF-κB）/NLRP3途径减轻香烟烟雾诱导的肺部炎症。这些发现通过证明EBN抑制TNFR1/NF-κB/NLRP3信号通路，为EBN对肺部的积极营养特性提供了理论证据，该通路可预防香烟烟雾诱导的肺部炎症的发展。
