内毒素对抗坏血酸摄取的抑制作用：血清因子介导的证据。

Inhibition of ascorbic acid uptake by endotoxin: evidence of mediation by serum factor(s).

作者信息

Aleo J J, Padh H

出版信息

Proc Soc Exp Biol Med. 1985 May;179(1):128-31. doi: 10.3181/00379727-179-1-rc1.

Abstract

The effect of bacterial endotoxin on the ascorbic acid uptake by 3T6 fibroblasts was studied. Endotoxin inhibited ascorbic acid uptake by fibroblasts in a dose dependent manner. The inhibition by endotoxin takes place only in the presence of unheated serum; decomplementing serum by heat inactivation at 56 degrees C for 30 minutes eliminates endotoxin's inhibitory effect on ascorbic acid uptake. The effect of endotoxin appears to be instantaneous since the inhibition seen in the cells without any preexposure was similar to the cells preexposed to endotoxin for up to 6 hours. Polymyxin B sulfate which is known to bind the lipid A portion of endotoxin did not reverse the inhibition of ascorbic acid uptake caused by endotoxin.

摘要

研究了细菌内毒素对3T6成纤维细胞摄取抗坏血酸的影响。内毒素以剂量依赖的方式抑制成纤维细胞摄取抗坏血酸。内毒素的抑制作用仅在未加热血清存在时发生；通过在56℃加热灭活30分钟使血清补体失活可消除内毒素对抗坏血酸摄取的抑制作用。内毒素的作用似乎是即时的，因为在未预先暴露的细胞中观察到的抑制作用与预先暴露于内毒素长达6小时的细胞相似。已知能结合内毒素脂质A部分的硫酸多粘菌素B并不能逆转内毒素引起的抗坏血酸摄取抑制作用。