Department of Medical Pathology, Ataturk Training and Research Hospital, Katip Celebi University, Izmir, Turkey.
Department of Medical Pathology, Mengucek Gazi Training and Research Hospital, Binali Yildirim University, Erzincan, Turkey.
Mol Biol Rep. 2024 Jun 14;51(1):756. doi: 10.1007/s11033-024-09663-7.
Dedifferentiated endometrial adenocarcinoma (DEAC) is a rare, aggressive subtype, accounting for 2% of all endometrial cancers. Poor survival in DEAC prompts the need for effective treatment modalities through better prognostic classification. MicroRNAs (miRNA) have essential roles in tumor angiogenesis, which might enable their use as novel biomarkers. In this study, we aimed to reveal the relationship between the expression of miRNA-21 and miRNA-143, which are associated with angiogenesis, and the prognosis of DEAC.
The study included six cases diagnosed with DEAC. The expression levels of miRNA-21 and miRNA-143 were detected by quantitative real-time PCR. Microvascular density (MVD) was measured by CD34 staining. All data and effects on survival were compared for statistical significance.
Six cases diagnosed with DEAC were included in the study. The percentage of undifferentiated components ranged from 50 to 90%. The second component of differentiated carcinoma was detected as endometrioid (3/5 grade I, 1/5 grade II, 1/5 grade III) in five cases and serous in one case. The mean MVD was 27 (range 17-44, SD 9.4). In three cases, miRNA-21 expression was down-regulated in neoplastic areas compared to non-neoplastic areas. On the contrary, it was found to be up-regulated in the remaining three cases. MiRNA-143 expression decreased in four cases and increased in two cases.
Based on these findings, we found a significant irregular expression of miRNA-21 in DEACs. As in other cancers, angiogenesis is significantly associated with survival in DEACs. This study provides initial data for revealing possible implications of miRNAs as prognostic indicators in DEAC.
去分化子宫内膜腺癌（DEAC）是一种罕见的侵袭性亚型，占所有子宫内膜癌的 2%。DEAC 的生存率较差，促使我们需要通过更好的预后分类来寻找有效的治疗方法。微小 RNA（miRNA）在肿瘤血管生成中具有重要作用，这可能使其成为新的生物标志物。在这项研究中，我们旨在揭示与血管生成相关的 miRNA-21 和 miRNA-143 的表达与 DEAC 预后之间的关系。
本研究纳入了 6 例 DEAC 患者。通过实时定量 PCR 检测 miRNA-21 和 miRNA-143 的表达水平。通过 CD34 染色测量微血管密度（MVD）。对所有数据和对生存的影响进行比较以确定统计学意义。
本研究纳入了 6 例 DEAC 患者。未分化成分的比例范围为 50%至 90%。在 5 例中，分化癌的第二成分检测为子宫内膜样（3/5 级 I，1/5 级 II，1/5 级 III），1 例为浆液性。平均 MVD 为 27（范围 17-44，SD 9.4）。在 3 例中，肿瘤区的 miRNA-21 表达低于非肿瘤区。相反，其余 3 例中发现 miRNA-21 表达上调。miRNA-143 的表达在 4 例中降低，在 2 例中升高。
基于这些发现，我们发现 DEAC 中 miRNA-21 的表达存在明显的异常。与其他癌症一样，血管生成与 DEAC 的生存率显著相关。本研究为揭示 miRNA 作为 DEAC 预后指标的可能意义提供了初步数据。
