Laboratorio de Bioconservación y Manejo, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Prolongación de Manuel Carpio y Plan de Ayala s/n, Col. Santo Tomás C.P. 11340 Alcaldía Miguel Hidalgo, Ciudad de México, México.
Department of Biological Sciences, Clemson University, 132 Long Hall, Clemson, SC, 29634, USA.
Mol Biol Rep. 2024 Jun 14;51(1):760. doi: 10.1007/s11033-024-09700-5.
The genus Corynorhinus is composed of four recognized species: C. rafinesquii, C. townsendii, C. mexicanus, and C. leonpaniaguae, the latter two being endemic to Mexico. According to the IUCN, C. mexicanus is considered "Near Threatened", as its populations are dwindling and habitats are affected by anthropogenic disturbance. Corynorhinus leonpaniaguae has not been assigned to an IUCN Red List risk category due to its recent description.
In this study, the mitochondrial genomes of C. mexicanus and C. leonpaniaguae were assembled and characterized in detail. The mitochondrial genomes (mtDNA) of C. mexicanus and C. leonpaniaguae have lengths of 16,470 and 16,581 bp respectively, with a predominant nucleotide usage of adenine (31.670% and 31.729%, respectively) and thymine (26.15% and 26.18%, respectively). The mtDNA of C. mexicanus and C. leonpaniaguae is composed of 37 coding and non-coding elements: 22 transfer RNAs (tRNA), 13 protein-coding genes (PCGs), two ribosomal RNAs and a non-coding region, the control region, which has a length of 933 bp and 1,149 bp, respectively. All tRNAs exhibited a cloverleaf secondary structure, with the exception of trn-Ser1 which showed a deletion of the dihydrouridine arm in the two species. All PCGs are subjected to purifying selection, with atp8 being the gene showing the highest Ka/Ks value.
These are the first whole mitogenomic resources developed for C. mexicanus and C. leonpaniaguae and enhance our knowledge of the ecology of these species and aid in their conservation.
Corynorhinus 属由四个公认的物种组成：C. rafinesquii、C. townsendii、C. mexicanus 和 C. leonpaniaguae，后两种物种仅分布于墨西哥。根据 IUCN 的标准，C. mexicanus 被认为是“近危”物种，因为其数量正在减少，栖息地受到人为干扰的影响。Corynorhinus leonpaniaguae 由于最近才被描述，因此尚未被列入 IUCN 红色名录风险类别。
在这项研究中，详细组装和描述了 C. mexicanus 和 C. leonpaniaguae 的线粒体基因组。C. mexicanus 和 C. leonpaniaguae 的线粒体基因组（mtDNA）长度分别为 16,470 和 16,581 bp，碱基使用以腺嘌呤（分别为 31.670%和 31.729%）和胸腺嘧啶（分别为 26.15%和 26.18%）为主。C. mexicanus 和 C. leonpaniaguae 的 mtDNA 由 37 个编码和非编码元件组成：22 个转移 RNA（tRNA）、13 个蛋白质编码基因（PCG）、两个核糖体 RNA 和一个非编码区，即控制区，长度分别为 933 bp 和 1,149 bp。所有 tRNA 都表现出三叶形二级结构，除了两种物种中的 trn-Ser1 缺失了二氢尿嘧啶臂。所有 PCG 都受到纯化选择的影响，其中 atp8 基因的 Ka/Ks 值最高。
这些是首次为 C. mexicanus 和 C. leonpaniaguae 开发的完整线粒体基因组资源，增强了我们对这些物种生态的认识，并有助于它们的保护。
