Laboratorio de Biocoservación y Manejo, Posgrado en Ciencias Químico-Biológicas, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación Carpio y Plan de Ayala s/n, Col. Santo Tomás, C.P. 11340, Ciudad de México, México; Laboratorio Ecología Evolutiva y Conservación. Departamento de Conservación de la Biodiversidad, ECOSUR-Villahermosa, Carretera Reforma Km 15.5, Ra. Guineo 2da. Sección, Villahermosa, México.
Laboratorio de Biocoservación y Manejo, Posgrado en Ciencias Químico-Biológicas, Departamento de Zoología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación Carpio y Plan de Ayala s/n, Col. Santo Tomás, C.P. 11340, Ciudad de México, México.
Gene. 2024 Aug 5;918:148492. doi: 10.1016/j.gene.2024.148492. Epub 2024 Apr 20.
In the species-rich family Vespertilionidae, vesper yellow bats in the genus Rhogeessa include eleven species, three of them endemic to Mexico. These insectivorous bats provide important ecosystem services, including pest control. Even though some aspects of their biology are well- known, only a few genomic resources are available for these species, which limits our understanding of their biology. In this study, we assembled and annotated the mitochondrial genome of four species: R. aenea, R. genowaysi, R. mira, and R. parvula. We generated a phylomitogenomic hypothesis based on translated protein-coding genes for a total of 52 species in the family Vespertilionidae and examined the phylogenetic position of the genus Rhogeessa and species within the family. The AT-rich mitogenomes of R. aenea, R. genowaysi, R. mira, and R. parvula are 16,763, 16,781, 16,807, and 16,794 pb in length, respectively. Each studied mitogenome encodes 13 Protein Coding Genes (PCGs), 22 transfer RNA genes, and 2 rRNA genes, and contains a putative control region (CR). All tRNAs exhibit a 'cloverleaf' secondary structure, except tRNA-Serine-1 that lacked the DHU arm in all studied mitogenomes. Selective pressure analyses indicated that all protein-coding genes are exposed to purifying selection. The phylomitogenomic analysis supported the monophyletic status of the family Vespertilionidae, confirmed the placement of Rhogeessa within the tribe Antrozoini, and clarified phylogenetic relationships within and among subfamilies and tribes in this family. Our results indicate that phylomitogenomics are useful to explore the evolutionary history of vesper bats. The assembly and comprehensive analysis of mitochondrial genomes offer the potential to generate molecular references and resources beneficial for genetic analyses aimed at understanding the ecology and evolution of these remarkable bats.
在物种丰富的蝙蝠科中，包括 Rhogeessa 属在内的 11 种食虫蝙蝠中，有 3 种是墨西哥特有的。这些食虫蝙蝠提供了重要的生态系统服务，包括害虫控制。尽管它们的一些生物学特征已经广为人知，但这些物种的基因组资源却非常有限，这限制了我们对它们生物学的理解。在这项研究中，我们组装并注释了四种蝙蝠的线粒体基因组：R. aenea、R. genowaysi、R. mira 和 R. parvula。我们基于翻译后的蛋白质编码基因为蝙蝠科的 52 种蝙蝠生成了一个 phylomitogenomic 假说，并研究了 Rhogeessa 属和蝙蝠科内物种的系统发育位置。R. aenea、R. genowaysi、R. mira 和 R. parvula 的富含 AT 的线粒体基因组分别长 16763、16781、16807 和 16794bp。每个研究的线粒体基因组编码 13 个蛋白编码基因（PCGs）、22 个转移 RNA 基因和 2 个 rRNA 基因，并包含一个推定的控制区（CR）。所有 tRNA 都表现出“三叶草”二级结构，除了所有研究的线粒体基因组中的 tRNA-Serine-1 缺乏 DHU 臂。选择压力分析表明，所有蛋白质编码基因都受到纯化选择的影响。Phylomitogenomic 分析支持蝙蝠科的单系性，证实了 Rhogeessa 属在 Antrozoini 部落中的位置，并阐明了该科内亚科和部落内以及之间的系统发育关系。我们的研究结果表明 phylomitogenomics 有助于探索食虫蝙蝠的进化历史。线粒体基因组的组装和综合分析为生成分子参考和资源提供了潜力，这些资源有利于遗传分析，有助于了解这些非凡蝙蝠的生态和进化。
