阳离子脂质纳米颗粒介导的 Cas9/crRNA 核糖核蛋白复合物转导实现柑橘植物基因组的无转基因编辑。
Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome.
机构信息
Department of Horticultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, USA.
Plant Breeding Graduate Program, University of Florida, Gainesville, FL, USA.
出版信息
Plant Cell Rep. 2024 Jun 14;43(7):171. doi: 10.1007/s00299-024-03254-3.
PMID:38874819
Abstract
A lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein (RNP) complex resulted in the production of transgene free genome edited citrus.
摘要
利用 Cas9/gRNA 核糖核蛋白 (RNP) 复合物的无 DNA 基因组编辑的脂转染方法，对柑橘原生质体细胞进行转染，可获得无转基因的基因组编辑柑橘。
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