Department of Neurobiology and Department of Affiliated Mental Health Center of Hangzhou Seventh People's Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 310000, China; MOE Frontier Science Center for Brain Science & Brain-Machine Integration, State Key Laboratory of Brain-machine Intelligence, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310000, China; NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University, Hangzhou 310000, China.
Department of Neurobiology and Department of Affiliated Mental Health Center of Hangzhou Seventh People's Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 310000, China; MOE Frontier Science Center for Brain Science & Brain-Machine Integration, State Key Laboratory of Brain-machine Intelligence, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310000, China; NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University, Hangzhou 310000, China.
STAR Protoc. 2024 Jun 21;5(2):103131. doi: 10.1016/j.xpro.2024.103131. Epub 2024 Jun 13.
To exclude the influence of motion on in vivo calcium imaging, animals usually need to be fixed. However, the whole-body restraint can cause stress in animals, affecting experimental results. In addition, some brain regions are prone to bleeding during surgery, which lowers the success rate of calcium imaging. Here, we present a protocol for calcium imaging using heparin-treated fiber in head-fixed mice. We describe steps for stereotaxic surgery, including virus injection and optic fiber implantation, fiber photometry, and data analysis. For complete details on the use and execution of this protocol, please refer to Du et al..
为了排除动物体内钙成像中运动的影响，通常需要将动物固定。然而，全身束缚会给动物带来压力，影响实验结果。此外，在手术过程中，一些脑区容易出血，这降低了钙成像的成功率。在这里，我们提出了一种在头部固定的小鼠中使用肝素处理光纤进行钙成像的方案。我们描述了立体定向手术的步骤，包括病毒注射和光纤植入、光纤光度测定和数据分析。有关此方案使用和执行的完整详细信息，请参考 Du 等人的研究。
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