Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Division of Bio-Medical Science & Technology, Korea University of Science and Technology (UST), Daejeon, Republic of Korea.
Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea; Program of Brain and Cognitive Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea.
STAR Protoc. 2024 Jun 21;5(2):102931. doi: 10.1016/j.xpro.2024.102931. Epub 2024 Mar 11.
In vivo calcium imaging of neural activity is an indispensable approach for understanding the mechanisms and functions of neural system. Development of advanced imaging tools and various genetically encoded calcium indicators allows us to simultaneously record the activity of different neural populations. Here, we present a protocol for acquiring neural activity of two discrete neural populations in mice using dual-color fiber photometry. We describe steps for injecting viral constructs and implanting the fiber optic through stereotaxic surgery, calcium signal acquisition, and data analysis. We also describe the incorporation of electroencephalogram and electromyography recordings with dual-color fiber photometry analysis. For complete details on the use and execution of this protocol, please refer to Shin et al..
在体钙成像技术是理解神经系统机制和功能不可或缺的手段。先进的成像工具和各种基因编码钙指示剂的发展使我们能够同时记录不同神经元群体的活动。本实验方案介绍了使用双色光纤光度法在小鼠中获取两个离散神经元群体的神经活动的方法。我们描述了通过立体定向手术注射病毒载体和植入光纤、钙信号采集和数据分析的步骤。我们还描述了将脑电图和肌电图记录与双色光纤光度法分析相结合的方法。如需了解该方案的详细使用和执行情况，请参考 Shin 等人的研究。
