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二态真菌白色念珠菌的分泌型磷脂酶;三种酶的分离及一些生物学特性

Secreted phospholipases of the dimorphic fungus, Candida albicans; separation of three enzymes and some biological properties.

作者信息

Banno Y, Yamada T, Nozawa Y

出版信息

Sabouraudia. 1985 Feb;23(1):47-54. doi: 10.1080/00362178585380081.

Abstract

Several phospholipases are secreted into the culture medium by growing yeast cells of Candida albicans 3125. DEAE-Sephadex column chromatography of concentrated culture filtrate revealed three separable fractions with phospholipase activities. Analysis of products of hydrolysis showed that the enzyme activities were lysophospholipase, lysophospholipase-transacylase and a phospholipase B.

摘要

白色念珠菌3125的酵母细胞在生长过程中会向培养基中分泌几种磷脂酶。对浓缩培养滤液进行二乙氨基乙基葡聚糖(DEAE-Sephadex)柱层析,结果显示具有磷脂酶活性的三个可分离组分。水解产物分析表明,这些酶活性分别为溶血磷脂酶、溶血磷脂酶转酰基酶和磷脂酶B。

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