Hirano K, Ito T, Morihara H, Tanaka T, Satouchi K
Department of Food Science and Technology, Fukuyama University, Hiroshima, Japan.
FEBS Lett. 1998 Oct 23;437(3):193-6. doi: 10.1016/s0014-5793(98)01226-5.
Phosphatidylcholine with docosahexaenoic acid at both sn-1 and sn-2 positions occurs in relatively high abundance in bonito muscle. To explore a possible route for the dipolyunsaturated molecular species, phosphatidylcholine formation from 2-[1-14C]linoleoyl lysophosphatidylcholine was examined using a cytosolic fraction from bonito muscle. The formation of radiolabeled phosphatidylcholine was greatest at 15 degrees C and did not require the presence of cofactors such as CoA and calcium. By DEAE-cellulofine column chromatography, the activity to form phosphatidylcholine was separated from that of phospholipase A1, and the specific activity increased by about 100-fold. The possible involvement of cytosolic lysophosphatidylcholine/transacylase in synthesis of dipolyunsaturated phosphatidylcholine is discussed.
在鲣鱼肌肉中，sn-1和sn-2位均含有二十二碳六烯酸的磷脂酰胆碱含量相对较高。为了探寻二多不饱和分子种类的可能途径，利用鲣鱼肌肉的胞质部分，研究了由2-[1-¹⁴C]亚油酰溶血磷脂酰胆碱生成磷脂酰胆碱的过程。放射性标记的磷脂酰胆碱在15℃时生成量最大，且不需要辅酶A和钙等辅助因子的存在。通过DEAE-纤维素细柱色谱法，将生成磷脂酰胆碱的活性与磷脂酶A1的活性分离，比活性提高了约100倍。本文讨论了胞质溶血磷脂酰胆碱/转酰基酶在二多不饱和磷脂酰胆碱合成中的可能作用。
