School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR. China.
College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
Bioresour Technol. 2024 Aug;406:130966. doi: 10.1016/j.biortech.2024.130966. Epub 2024 Jun 13.
This study constructed an integrated algae/partial nitrification/anammox biofilm system and operated it for 240 days. The total nitrogen removal efficiency exceeded 90 %. The structure, compositions, and function of this symbiotic biofilm, which played a pivotal role in the system, were analyzed in detail. Microscope photos and fluorescence in situ hybridization both showed that bacteria and algae were well integrated. The dissolved oxygen gradient further confirmed that different functional microorganisms grew at varying depths within biofilm. Algae formed an oxygen-producing zone (0-0.48 mm), followed by ammonia oxidizing bacteria (AOB) consuming oxygen to form an oxygen-consuming zone (0.48-0.86 mm), and anaerobic ammonia oxidizing bacteria (AnAOB) removed nitrogen in anaerobic zone (>0.86 mm). Chlorella, Nitrosomonas and Candidatus_Kuenenia were identified as the dominant algae, AOB and AnAOB, with relative abundances of 11.80 %, 19.77 % and 3.07 %, respectively. This layered biofilm benefitted providing a suitable environment for various microorganisms to survive within a complex biofilm.
本研究构建了一个藻类/部分硝化/厌氧氨氧化共生生物膜系统并运行了 240 天。总氮去除效率超过 90%。详细分析了在系统中起关键作用的这种共生生物膜的结构、组成和功能。显微镜照片和荧光原位杂交都表明细菌和藻类很好地结合在一起。溶解氧梯度进一步证实了不同功能的微生物在生物膜内的不同深度生长。藻类形成产氧区（0-0.48mm），随后氨氧化细菌（AOB）消耗氧气形成耗氧区（0.48-0.86mm），厌氧氨氧化菌（AnAOB）在厌氧区（>0.86mm）去除氮。小球藻、亚硝化单胞菌和卡氏菌被鉴定为优势藻类、AOB 和 AnAOB，相对丰度分别为 11.80%、19.77%和 3.07%。这种分层生物膜有利于为各种微生物在复杂生物膜内的生存提供合适的环境。
