Wang Yang-Yan, Cheng Hai-Xiang, Wang Pin, Fan Rui, Luo Long-Zao, Lin Guo-Hui, Tian Guang-Ming
College of Chemical and Material Engineering, Quzhou University, 78 North Jiuhua Road, Quzhou, 324000, People's Republic of China.
College of Environmental and Resource Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, People's Republic of China.
Sci Rep. 2025 Jul 2;15(1):22876. doi: 10.1038/s41598-025-06352-8.
Microalgae-bacteria consortia is an effective bioremediation technique employed for treating piggery effluent, and how to enhance its purification performance has always been a research hotspot. This study aims to investigate the growth and biological state of microalgae-bacteria consortia during swine wastewater treatment doped with nano-sized iron including Nano-ZVI and Nano-FeO. Results showed that the addition of nano-sized iron was more conducive to microalgal growth and pollutant removal from swine wastewater, especially the addition of Nano-ZVI, in which the microalgal biomass was 31.14% higher than that of the control, and the removal rates of NH-N, TN, TP and COD increased to 99.55%, 61.57%, 91.86%, and 49.24%, respectively. The removal rates of Cu and Zn were 93.12% and 99.89%, respectively, after four days of treatment. The addition of Nano-ZVI significantly increased the relative abundance of Bacteroidetes and functional genera, such as Flavobacterium, Brevundimonas, Bacillus, and Hydrogenophaga. 8 metabolic pathways were significantly upregulated compared with the control, encompassing important biochemical processes such as biosynthesis, signal transduction, and energy production, which are closely related to microalgal activity and nutrient absorption. These findings provide scientific guidance for the enhance of microalgae-bacterial consortia in wastewater treatment doped with nano-sized iron.
微藻-细菌共生体是一种用于处理猪场废水的有效生物修复技术，如何提高其净化性能一直是研究热点。本研究旨在探究在掺杂纳米铁（包括纳米零价铁和纳米氧化铁）的猪废水处理过程中微藻-细菌共生体的生长和生物学状态。结果表明，添加纳米铁更有利于微藻生长和猪废水中污染物的去除，尤其是添加纳米零价铁，其中微藻生物量比对照组高31.14%，NH-N、TN、TP和COD的去除率分别提高到99.55%、61.57%、91.86%和49.24%。处理四天后，Cu和Zn的去除率分别为93.12%和99.89%。添加纳米零价铁显著提高了拟杆菌门以及黄杆菌属、短波单胞菌属、芽孢杆菌属和嗜氢菌属等功能菌属的相对丰度。与对照组相比，8条代谢途径显著上调，包括生物合成、信号转导和能量产生等重要生化过程，这些过程与微藻活性和养分吸收密切相关。这些发现为在掺杂纳米铁的废水处理中强化微藻-细菌共生体提供了科学指导。
