College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.
Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, 02129, USA.
Reprod Biol Endocrinol. 2024 Jun 14;22(1):67. doi: 10.1186/s12958-024-01239-1.
This comprehensive review explores the evolving landscape of sperm selection techniques within the realm of Assisted Reproductive Technology (ART). Our analysis delves into a range of methods from traditional approaches like density gradient centrifugation to advanced techniques such as Magnetic-Activated Cell Sorting (MACS) and Intracytoplasmic Morphologically Selected Sperm Injection (IMSI). We critically assess the efficacy of these methods in terms of sperm motility, morphology, DNA integrity, and other functional attributes, providing a detailed comparison of their clinical outcomes. We highlight the transition from conventional sperm selection methods, which primarily focus on physical characteristics, to more sophisticated techniques that offer a comprehensive evaluation of sperm molecular properties. This shift not only promises enhanced prediction of fertilization success but also has significant implications for improving embryo quality and increasing the chances of live birth. By synthesizing various studies and research papers, we present an in-depth analysis of the predictability of different sperm selection procedures in ART. The review also discusses the clinical applicability of these methods, emphasizing their potential in shaping the future of assisted reproduction. Our findings suggest that the integration of advanced sperm selection strategies in ART could lead to more cost-effective treatments with reduced duration and higher success rates. This review aims to provide clinicians and researchers in reproductive medicine with comprehensive insights into the current state and future prospects of sperm selection technologies in ART.
本综述全面探讨了辅助生殖技术（ART）领域中精子选择技术的发展现状。我们的分析深入研究了一系列方法，包括传统方法如密度梯度离心法，以及先进技术如磁激活细胞分选（MACS）和胞浆内形态选择精子注射（IMSI）。我们从精子活力、形态、DNA 完整性和其他功能特性等方面评估了这些方法的效果，详细比较了它们的临床结局。我们强调了从主要关注物理特征的传统精子选择方法向更复杂的技术的转变，这些技术提供了对精子分子特性的全面评估。这种转变不仅有望提高受精成功率的预测能力，而且对改善胚胎质量和提高活产率具有重要意义。通过综合各种研究和研究论文，我们对 ART 中不同精子选择程序的可预测性进行了深入分析。该综述还讨论了这些方法的临床适用性，强调了它们在塑造辅助生殖未来方面的潜力。我们的研究结果表明，将先进的精子选择策略整合到 ART 中可能会导致更具成本效益的治疗方法，治疗时间更短，成功率更高。本综述旨在为生殖医学领域的临床医生和研究人员提供有关 ART 中精子选择技术的现状和未来前景的全面见解。
