采用导数紫外分光光度法测定药物制剂中左氧氟沙星、诺氟沙星和莫西沙星或单独测定这些药物。
Determination of levofloxacin, norfloxacin, and moxifloxacin in pharmaceutical dosage form or individually using derivative UV spectrophotometry.
作者信息
Elgendy K H, Zaky M, Altorky Alaa Eldin Mohamed Mahmoud, Fadel S
机构信息
Chemistry Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
出版信息
BMC Chem. 2024 Jun 14;18(1):115. doi: 10.1186/s13065-024-01193-4.
PURPOSE
In this study, first, second, third, and fourth-order derivative spectrophotometric methods utilizing the peak-zero (P-O) and peak-peak (P-P) techniques of measurement were developed for the determination of levofloxacin, norfloxacin, and moxifloxacin. These methods were applied to their combined pharmaceutical dosage form or individually for levofloxacin, norfloxacin, and moxifloxacin.
METHODS
Linearity was established in the concentration range of 2-20 µg/mL. The procedures are simple, quick, and precise. The developed methods are sensitive, accurate, and cost-effective, demonstrating excellent correlation coefficients (R2 = 0.9998) and mean recovery values ranging from 99.20% to 100.08%, indicating a high level of precision.
RESULTS
The developed approach was effectively employed to determine the levofloxacin, norfloxacin, and moxifloxacin content in commercially available pharmaceutical dosages.
CONCLUSIONS
Statistical analysis and recovery tests confirmed the method's linearity and accuracy. The results suggest that this method can be utilized for routine analysis in both bulk and commercial formulations. The simplicity, accuracy, and cost-effectiveness of the developed methods make them valuable for pharmaceutical analysis.
目的
在本研究中，开发了利用峰零（P - O）和峰峰（P - P）测量技术的一阶、二阶、三阶和四阶导数分光光度法，用于测定左氧氟沙星、诺氟沙星和莫西沙星。这些方法应用于它们的复方药物剂型，或单独用于左氧氟沙星、诺氟沙星和莫西沙星。
方法
在2 - 20μg/mL的浓度范围内建立线性关系。这些程序简单、快速且精确。所开发的方法灵敏、准确且具有成本效益，显示出优异的相关系数（R2 = 0.9998），平均回收率在99.20%至100.08%之间，表明精密度较高。
结果
所开发的方法有效地用于测定市售药物剂型中左氧氟沙星、诺氟沙星和莫西沙星的含量。
结论
统计分析和回收率测试证实了该方法的线性和准确性。结果表明该方法可用于原料药和商业制剂的常规分析。所开发方法的简单性、准确性和成本效益使其在药物分析中具有价值。
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