School of Chemistry and Chemical Engineering, Gannan Normal University, Ganzhou, 341000, China.
Department of Chemistry, Nanchang University, Nanchang, 330031, China.
Anal Bioanal Chem. 2024 Nov;416(27):6045-6055. doi: 10.1007/s00216-024-05390-x. Epub 2024 Jun 15.
As a representative gas of food spoilage, the development of rapid hydrogen sulfide (HS) analysis strategies for food safety control is in great demand. Despite traditional methods for HS detection possessing great achievements, they are still incapable of meeting the requirement of portability and quantitative detection at the same time. Herein, a nanozyme catalysis pressure-powered sensing platform that enables visual quantification with the naked eye is proposed. In this methodology, Pt nanozyme inherits the catalase-like activity to facilitate the decomposition of HO to O, which can significantly improve the pressure in the closed container, further pushing the movement of indicator dye. Furthermore, HS was found to effectively inhibit the catalytic activity of Pt nanozyme, indicating that the catalase-like activity of PtNPs may be regulated by varying concentrations of HS. Therefore, by utilizing a self-designed pressure-powered microchannel device, the concentration of HS was successfully converted into a distinct signal variation in distance. The effectiveness of the as-designed sensor in assessing the spoilage of red wine by HS determination has been demonstrated. It exhibits a strong correlation between the change in dye distance and HS concentration within the range of 1-250 μM, with a detection limit of 0.17 μM. This method is advantageous as it enhances the quantitative detection of HS with the naked eye based on the portable pressure-powered sensing platform, as compared to traditional HS biosensors. Such a pressure-powered distance variation platform would greatly broaden the application of HS-based detection in food spoilage management.
作为食品变质的代表性气体，快速分析食品安全控制中硫化氢（HS）的发展需求巨大。尽管传统的 HS 检测方法已经取得了很大的成就，但它们仍然不能同时满足便携性和定量检测的要求。本文提出了一种纳米酶催化压力传感平台，该平台可实现肉眼可视化定量检测。在该方法中，Pt 纳米酶继承了类过氧化物酶的活性，促进了 HO 分解为 O，这可以显著提高封闭容器中的压力，进一步推动指示剂染料的运动。此外，HS 被发现可以有效抑制 Pt 纳米酶的催化活性，表明 PtNPs 的类过氧化物酶活性可以通过改变 HS 的浓度进行调节。因此，通过使用自行设计的压力驱动微流控装置，成功地将 HS 浓度转换为明显的距离信号变化。该设计的传感器在通过 HS 测定评估红酒变质方面的有效性已经得到了证明。它在 1-250 μM 的范围内表现出染料距离变化与 HS 浓度之间的强烈相关性，检测限为 0.17 μM。与传统的 HS 生物传感器相比，这种基于便携式压力驱动传感平台的增强型肉眼定量检测 HS 的方法具有优势。这种压力驱动距离变化平台将极大地拓宽基于 HS 的检测在食品变质管理中的应用。
