School of Chemistry and Chemical Engineering, School of Medicine, Zhenjiang 212013, PR China.
School of Chemistry and Chemical Engineering, School of Medicine, Zhenjiang 212013, PR China.
J Hazard Mater. 2024 Mar 5;465:133036. doi: 10.1016/j.jhazmat.2023.133036. Epub 2023 Nov 22.
A highly efficient system incorporates the real-time visualization of the two toxic molecules (HS and NH) and the recognition of corresponding transforms using a fluorescent sensor. In this paper, a dual-responsive probe (QS-DNP) based on methylquinolinium-salicyaldehyde-2,4-dinitrophenyl was developed that can simultaneously detect HS and NH at two independent fluorescent channels without signal crosstalk. QS-DNP showed excellent anti-interference, high selectivity, outstanding water solubility, low LOD values (HS: 51 nM; NH: 40 nM), low cytotoxicity, and mitochondrial localization properties. The 2,4-dinitrophenyl site was sensitive to HS, and the CC bridge was reactive to NH, with strong fluorescence at 680 and 488 nm, respectively. The wavelength gap between these two channels is 192 nm; verify that there is no signal crosstalk throughout detection. By this means, the probe was used to simultaneously detect HS and NH in real soil samples, food samples, and living cells. The endogenous HS and NH were monitored in HeLa cells and investigated the mitochondria organelle of living cells with a positive charge on QS-DNP. Overall, all results emphasize that the QS-DNP probe is a powerful tool for the simultaneous detection of HS and NH and presents a potential new sensing approach.
一个高效的系统结合了实时可视化的两个有毒分子（HS 和 NH）和识别相应变换使用荧光传感器。在本文中，一个双重响应探针（QS-DNP）基于甲基喹啉-salicyaldehyde-2,4-二硝基苯的开发可以同时检测 HS 和 NH 在两个独立的荧光通道没有信号串扰。QS-DNP 表现出良好的抗干扰能力，高选择性，出色的水溶性，低检测限值（HS：51 nM; NH：40 nM），低细胞毒性和线粒体定位性能。2,4-二硝基苯基部位对 HS 敏感，CC 桥对 NH 反应，分别在 680 和 488nm 处有强荧光。这两个通道之间的波长间隙为 192nm；验证在整个检测过程中没有信号串扰。通过这种方式，探针被用于同时检测实际土壤样品、食品样品和活细胞中的 HS 和 NH。内源性 HS 和 NH 在 HeLa 细胞中进行了监测，并研究了带有正电荷的 QS-DNP 的活细胞线粒体器官。总的来说，所有的结果都强调 QS-DNP 探针是一种强大的工具，用于同时检测 HS 和 NH，并提出了一种潜在的新的传感方法。
