Liozner A L, Stepanova E N, Volynskaia N A, Vorkunova G K, Bukrinskaia A G
Zh Mikrobiol Epidemiol Immunobiol. 1985 Mar(3):91-6.
A test system permitting the detection of influenza virus protein M at a concentration of 0.1-0.5 ng/ml in ELISA has been developed. The use of this system made it possible to detect influenza viruses A and B directly in crude virus-containing material and clinical samples obtained from influenza patients. During the outbreak of influenza in the spring of 1983 ELISA was successfully used for the rapid diagnosis of influenza, and some of its advantages in comparison with the conventional immunofluorescence test were thus demonstrated. To overcome difficulties arising from the low immunogenic potency of protein M, in the process of obtaining diagnostic sera and ascitic fluids the animals were immunized with the conjugate of protein M and polyelectrolite, which ensured considerable activation of humoral immune response.
已开发出一种检测系统，该系统在酶联免疫吸附测定（ELISA）中能够检测浓度为0.1 - 0.5纳克/毫升的流感病毒蛋白M。使用该系统可直接在含粗病毒的材料以及从流感患者获取的临床样本中检测甲型和乙型流感病毒。在1983年春季流感爆发期间，ELISA成功用于流感的快速诊断，从而证明了其与传统免疫荧光试验相比的一些优势。为克服因蛋白M免疫原性低而产生的困难，在获取诊断血清和腹水的过程中，用蛋白M与聚电解质的缀合物对动物进行免疫，这确保了体液免疫反应的显著激活。
