Bukrinskaia A G, Vorkunova G K, Liozner A L
Vopr Virusol. 1984 Nov-Dec;29(6):679-82.
Antigenic properties of M1 proteins of influenza B viruses were studied by indirect EIA using monospecific antisera to M1 protein obtained by immunization of rabbits and mice. Antisera with highest titres were obtained by immunization of mice with polyelectrolyte-conjugated M1 protein. Antigenic heterogeneity of M1 proteins of influenza B viruses was demonstrated. M1 proteins of B/Lee/40 and B/USSR/03/84 viruses reacted with B/Hong Kong/72 antiserum to one-fourth of the homologous titre. Cross-experiments using M1 proteins recovered from influenza type A and B viruses and antisera to them revealed heterologous reactions suggesting a common antigenic determinant in A and B M1 proteins. Treatment of the antisera with M1 protein of type A resulted in a decline of antibody titres to M1 proteins of type B. The two available monoclones to M1 protein of type A reacted only with M1 A but not with M1 B.
利用通过免疫兔和小鼠获得的针对M1蛋白的单特异性抗血清，通过间接酶免疫测定法研究了乙型流感病毒M1蛋白的抗原特性。用聚电解质偶联的M1蛋白免疫小鼠可获得效价最高的抗血清。证明了乙型流感病毒M1蛋白的抗原异质性。B/Lee/40和B/USSR/03/84病毒的M1蛋白与B/香港/72抗血清反应，反应效价为同源效价的四分之一。使用从甲型和乙型流感病毒中回收的M1蛋白及其抗血清进行的交叉实验显示出异源反应，表明甲型和乙型M1蛋白中存在共同的抗原决定簇。用甲型M1蛋白处理抗血清导致针对乙型M1蛋白的抗体效价下降。两种现有的针对甲型M1蛋白的单克隆抗体仅与甲型M1反应，而不与乙型M1反应。
