Kurtuldu Fatih, Mutlu Nurshen, Friedrich Ralf P, Beltrán Ana M, Liverani Liliana, Detsch Rainer, Alexiou Christoph, Galusek Dušan, Boccaccini Aldo R
FunGlass, Alexander Dubček University of Trenčín, 911 50 Trenčín, Slovakia; Institute of Biomaterials, Department of Materials Science and Engineering, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91058 Erlangen, Germany.
Department of Otorhinolaryngology, Section for Experimental Oncology and Nanomedicine (SEON), Else Kröner-Fresenius-Stiftung-Professorship, Universitätsklinikum Erlangen, 91054 Erlangen, Germany.
Biomater Adv. 2024 Sep;162:213922. doi: 10.1016/j.bioadv.2024.213922. Epub 2024 Jun 5.
Mesoporous silica nanoparticles were synthesized using a microemulsion-assisted sol-gel method, and calcium, gallium or a combination of both, were used as dopants. The influence of these metallic ions on the physicochemical properties of the nanoparticles was investigated by scanning and transmission electron microscopy, as well as N adsorption-desorption methods. The presence of calcium had a significant impact on the morphology and textural features of the nanoparticles. The addition of calcium increased the average diameter of the nanoparticles from 80 nm to 150 nm, while decreasing their specific surface area from 972 m/g to 344 m/g. The nanoparticles of all compositions were spheroidal, with a disordered mesoporous structure. An ion release study in cell culture medium demonstrated that gallium was released from the nanoparticles in a sustained manner. In direct contact with concentrations of up to 100 μg/mL of the nanoparticles, gallium-containing nanoparticles did not exhibit cytotoxicity towards pre-osteoblast MC3T3-E1 cells. Moreover, in vitro cell culture tests revealed that the addition of gallium to the nanoparticles enhanced osteogenic activity. Simultaneously, the nanoparticles disrupted the osteoclast differentiation of RAW 264.7 macrophage cells. These findings suggest that gallium-containing nanoparticles possess favorable physicochemical properties and biological characteristics, making them promising candidates for applications in bone tissue regeneration, particularly for unphysiological or pathological conditions such as osteoporosis.
采用微乳液辅助溶胶-凝胶法合成了介孔二氧化硅纳米颗粒，并使用钙、镓或两者的组合作为掺杂剂。通过扫描电子显微镜、透射电子显微镜以及N吸附-脱附方法研究了这些金属离子对纳米颗粒物理化学性质的影响。钙的存在对纳米颗粒的形态和结构特征有显著影响。添加钙使纳米颗粒的平均直径从80 nm增加到150 nm，同时其比表面积从972 m²/g降低到344 m²/g。所有组成的纳米颗粒均为球形，具有无序的介孔结构。细胞培养基中的离子释放研究表明，镓以持续的方式从纳米颗粒中释放出来。在与浓度高达100 μg/mL的纳米颗粒直接接触时，含镓纳米颗粒对前成骨细胞MC3T3-E1细胞未表现出细胞毒性。此外，体外细胞培养试验表明，向纳米颗粒中添加镓可增强成骨活性。同时，纳米颗粒破坏了RAW 264.7巨噬细胞的破骨细胞分化。这些发现表明，含镓纳米颗粒具有良好的物理化学性质和生物学特性，使其成为骨组织再生应用的有前途的候选者，特别是对于骨质疏松症等非生理性或病理性疾病。
