Citrus Research Institute, National Engineering Research Center for Citrus, Southwest University, Chongqing 400712, China.
J Agric Food Chem. 2024 Apr 3;72(13):7010-7020. doi: 10.1021/acs.jafc.4c00732. Epub 2024 Mar 26.
Cyetpyrafen is a recently developed acaricide. The citrus red mite, (McGregor), has developed significant resistance to cyetpyrafen. However, the molecular mechanism underlying the cyetpyrafen resistance in remains unclear. Glutathione S-transferases (GSTs) play a critical role in arthropod pesticide resistance. This study showed that GSTs were potentially related to the resistance of to cyetpyrafen through synergistic experiments and enzyme activity analysis. An omega-family GST gene, was significantly up-regulated in the egg, nymph, and adult stages of the cyetpyrafen-resistant strain. Additionally, silencing of significantly increased the mortality of to cyetpyrafen and recombinant PcGSTO1 demonstrated the ability to metabolize cyetpyrafen. Our results indicated that the overexpression of is associated with cyetpyrafen resistance in , and they also provided valuable information for managing resistance in
噻嗪酮是一种新开发的杀螨剂。柑橘全爪螨（McGregor）已对噻嗪酮产生了显著的抗药性。然而，其抗药性的分子机制尚不清楚。谷胱甘肽 S-转移酶（GSTs）在节肢动物杀虫剂抗性中起着关键作用。本研究通过协同实验和酶活性分析表明，GSTs 可能与柑橘全爪螨对噻嗪酮的抗性有关。一个 omega 家族的 GST 基因，在噻嗪酮抗性品系的卵、若虫和成虫阶段显著上调。此外，沉默 显著增加了 对噻嗪酮的死亡率，并且重组 PcGSTO1 表现出代谢噻嗪酮的能力。我们的结果表明，在 中， 的过表达与噻嗪酮抗性有关，它们还为管理 中的抗性提供了有价值的信息。
