Identification and Functional Characterization of an Omega-Class Glutathione Gene Associated with Cyetpyrafen Resistance in (McGregor).

Cyetpyrafen is a recently developed acaricide. The citrus red mite, (McGregor), has developed significant resistance to cyetpyrafen. However, the molecular mechanism underlying the cyetpyrafen resistance in remains unclear. Glutathione S-transferases (GSTs) play a critical role in arthropod pesticide resistance. This study showed that GSTs were potentially related to the resistance of to cyetpyrafen through synergistic experiments and enzyme activity analysis. An omega-family GST gene, was significantly up-regulated in the egg, nymph, and adult stages of the cyetpyrafen-resistant strain. Additionally, silencing of significantly increased the mortality of to cyetpyrafen and recombinant PcGSTO1 demonstrated the ability to metabolize cyetpyrafen. Our results indicated that the overexpression of is associated with cyetpyrafen resistance in , and they also provided valuable information for managing resistance in