Singh V, Singh O, Kaul S, Talwar G P
J Steroid Biochem. 1985 Nov;23(5B):801-2. doi: 10.1016/s0022-4731(85)80017-0.
A monkey and a baboon immunized with GnRH-tetanus toxoid conjugate developed high anti-GnRH antibody titres which resulted in disruption of cyclicity and low estradiol and progesterone levels indicative of the inhibition of follicular development and ovulation. Sera of both animals reacted with GnRH(NH2) but were devoid of reactivity with peptide sequences, 4-6, 7-10 and 4-10 of GnRH as well as GnRH free acid. Both sera were however reactive with GnRH-Lys-muramyl dipeptide analogue and GnRH-Ala-Ala-Thr-Lys-Pro-Arg-OH. As these compounds differ from GnRH-free acid by the presence of amide linkage at C-terminus of GnRH, these studies point to the importance of the conformation involving amide group at this position for immunoreactivity.
用促性腺激素释放激素-破伤风类毒素偶联物免疫的一只猴子和一只狒狒产生了高抗促性腺激素释放激素抗体滴度，这导致了周期紊乱以及雌二醇和孕酮水平降低，表明卵泡发育和排卵受到抑制。两只动物的血清都与促性腺激素释放激素（NH2）发生反应，但与促性腺激素释放激素的4-6、7-10和4-10肽序列以及游离酸形式的促性腺激素释放激素没有反应。然而，两种血清都与促性腺激素释放激素-赖氨酸-胞壁酰二肽类似物和促性腺激素释放激素-丙氨酸-丙氨酸-苏氨酸-赖氨酸-脯氨酸-精氨酸-OH发生反应。由于这些化合物与游离酸形式的促性腺激素释放激素的不同之处在于促性腺激素释放激素C末端存在酰胺键，这些研究表明该位置涉及酰胺基团的构象对于免疫反应性很重要。
