植物源食品中甾体糖苷生物碱监测的综合方法。

A comprehensive approach to the monitoring of steroidal glycoalkaloids in foods of plant origin.

机构信息

Laboratory of Natural Product Analysis, Department of Environmental Analysis, Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland.

出版信息

Food Chem. 2024 Nov 1;457:140091. doi: 10.1016/j.foodchem.2024.140091. Epub 2024 Jun 13.

DOI:10.1016/j.foodchem.2024.140091

PMID:38879959

Abstract

Steroidal glycoalkaloids (GAs) are toxins produced by solanaceous plants. As there are no fully standardized methods for their extraction and determination in food, the research aimed to: (1) develop and critically compare methods based on gas (GC) and liquid (LC) chromatography, including their coupling with mass spectrometry, and (2) to develop and optimize a universal GA extraction method. Hyphenated techniques proved to be the most useful in GA analysis: LC-MS was the most sensitive one, while GC-MS offered the highest chromatographic resolution. It was proven that quantitative results obtained using different analytical techniques cannot be directly compared. New extraction method that is more efficient than the AOAC method (997.13) was then designed and optimized. It was characterized by higher absolute recovery (99% and 34%, respectively) and allowed to extract much more GAs from the same material (e.g. 21.2 ± 1.4 and 11.82 ± 0.97 mg g of potato tubers, respectively).

摘要

甾体糖苷生物碱（GAs）是茄科植物产生的毒素。由于目前在食品中没有完全标准化的提取和测定方法，因此本研究旨在：（1）开发和批判性地比较基于气相（GC）和液相（LC）色谱法的方法，包括与质谱法的联用，以及（2）开发和优化一种通用的 GA 提取方法。联用技术在 GA 分析中被证明是最有用的：LC-MS 是最灵敏的方法，而 GC-MS 则提供了最高的色谱分辨率。事实证明，使用不同分析技术获得的定量结果不能直接进行比较。然后设计并优化了一种比 AOAC 方法（997.13）更有效的新提取方法。该方法的特点是绝对回收率更高（分别为 99%和 34%），并且可以从相同的材料中提取更多的 GAs（例如，马铃薯块茎中的 21.2±1.4 和 11.82±0.97mg·g）。