Shakya Roshani, Navarre Duroy A
Department of Horticulture, Washington State University, Prosser, Washington 99350, USA.
J Agric Food Chem. 2006 Jul 26;54(15):5253-60. doi: 10.1021/jf0605300.
Evaluation of phenolic metabolism in potato tubers (Solanum tuberosum) would be facilitated by faster analytical methods. A high-throughput HPLC method was developed for the qualitative and quantitative determination in potato of numerous phenolic compounds, the sum of the glycoalkaloids chaconine and solanine, plus ascorbic acid. Following a fast extraction, HPLC run times of 12 min were achieved with the use of a monolithic RP C18 column. UV and MS analyses were used to characterize the phenolic complement in extracts from two white-fleshed varieties. Over 30 compounds were identified, some of which are thought to possess either nutritional value or are involved in plant disease resistance. This method is expected to be useful for germplasm mining and for varietal development programs in which large numbers of lines are generated.
更快的分析方法将有助于评估马铃薯块茎（Solanum tuberosum）中的酚类代谢。开发了一种高通量HPLC方法，用于定性和定量测定马铃薯中的多种酚类化合物、茄碱和龙葵碱糖苷生物碱的总和以及抗坏血酸。经过快速提取后，使用整体式反相C18柱可实现12分钟的HPLC运行时间。采用紫外和质谱分析对两个白肉品种提取物中的酚类成分进行表征。鉴定出30多种化合物，其中一些被认为具有营养价值或参与植物抗病性。预计该方法将有助于种质挖掘以及涉及大量品系的品种开发计划。
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