Vega C A, Buening G M, Green T J, Carson C A
Am J Vet Res. 1985 Feb;46(2):416-20.
A strain of Babesia bigemina was isolated from an infected calf and propagated in vitro. Culture conditions included washing of infected and normal bovine erythrocytes in a special solution, and the use of a 5% to 10% (v/v) erythrocyte suspension in medium 199 (with 20% to 50% fresh normal bovine serum) at a depth of 4 mm in a 5% CO2, 2% O2, 93% N2 atmosphere. After 36 days in vitro and 9 subcultures, the cultured organism was inoculated into a susceptible calf. This calf developed clinical signs of disease and recovered when treated with 1% trypan blue solution. The strain was also reisolated from the second calf. The original isolate had been maintained in continuous in vitro cultivation for more than 99 days.
从一头感染的小牛中分离出一株双芽巴贝斯虫，并在体外进行繁殖。培养条件包括用一种特殊溶液洗涤感染的和正常的牛红细胞，以及在含有20%至50%新鲜正常牛血清的199培养基中使用5%至10%（v/v）的红细胞悬液，在5%二氧化碳、2%氧气、93%氮气的气氛中于4毫米深度培养。在体外培养36天并传代9次后，将培养的生物体接种到一头易感小牛体内。这头小牛出现了疾病的临床症状，并用1%的台盼蓝溶液治疗后康复。该菌株也从第二头小牛中重新分离出来。原始分离株已在体外连续培养超过99天。
