Erp E E, Smith R D, Ristic M, Osorno B M
Am J Vet Res. 1980 Jul;41(7):1141-2.
Babesia bovis was isolated from an experimentally infected calf (No. 1) and was maintained in vitro for 32 days by subculturing 14 times, using a total dilution of 192,000. A splenectomized calf was inoculated with subculture Babesia (isolate B). The agent (isolate C) was isolated and maintained in vitro for 17 days prior to inoculation of a third splenectomized calf. Babesia organisms (isolate D) were isolated and retained in vitro for 270 days. After 85 days in vitro, organisms from this third isolation (isolate D) were injected into a fourth splenectomized calf to test for infectivity. Responses of the three calves to cultural inoculation were similar to those responses that occurred from inoculation of infected bovine blood (calf 1). There was no observed change in the microscopic appearance of the Babesia in vitro with time.
从一头经实验感染的小牛（1号）中分离出牛巴贝斯虫，并通过14次传代培养在体外维持32天，总稀释度为192,000。给一头脾切除的小牛接种传代培养的巴贝斯虫（分离株B）。在给第三头脾切除的小牛接种之前，分离出病原体（分离株C）并在体外维持17天。分离出巴贝斯虫生物体（分离株D）并在体外保存270天。在体外培养85天后，将第三次分离得到的生物体（分离株D）注射到第四头脾切除的小牛体内以检测其感染性。三头小牛对培养物接种的反应与接种感染牛血（小牛1）后的反应相似。随着时间的推移，体外观察到的巴贝斯虫微观形态没有变化。
