Zhang Zhongjing Chinese Medical Research Institute, Nanyang Medical College, Nanyang, China; Fujian Agriculture and Forestry University, Fuzhou, China.
Henan Province Key Laboratory of Zhang Zhongjing Formulae and Herbs for Immunoregulation, Nanyang Institute of Technology, Nanyang, China; State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Plant Sci. 2024 Sep;346:112158. doi: 10.1016/j.plantsci.2024.112158. Epub 2024 Jun 14.
Artemisia argyi is an herbaceous plant of the genus Artemisia. Its young and mature leaves are used as food and medicine, respectively. Glandular trichomes (GTs) are distributed on the leaf surface in A. argyi and are generally considered the location of flavonoid biosynthesis and accumulation. However, the mechanism of flavonoid biosynthesis and accumulation in A. argyi remains unclear. In this study, the coregulatory genes involved in flavonoid biosynthesis and trichome development in this species were screened and evaluated, and the biosynthetic pathways for key flavonoids in A. argyi were uncovered. AaMYB1 and AaYABBY1 were screened using weighted gene co-expression network analysis, and both genes were then genetically transformed into Nicotiana tabacum L. cv. K326 (tobacco). Simultaneously, AaYABBY1 was also genetically transformed into Arabidopsis thaliana. The total flavonoid and rutin contents were increased in tobacco plants overexpressing AaMYB1 and AaYABBY1, and the expression levels of genes participating in the flavonoid synthesis pathway, such as PAL, FLS, and F3H, were significantly up-regulated in plants overexpressing these genes. These results indicated that AaMYB1 and AaYABBY1 promote flavonoid biosynthesis in tobacco. Furthermore, compared to that in the wild-type, the trichome density was significantly increased in tobacco and A. thaliana plants overexpressing AaYABBY1. These results confirm that AaYABBY1 might be involved in regulating trichome formation in A. argyi. This indicates the potential genes involved in and provides new insights into the development of trichome cellular factories based on the "development-metabolism" interaction network and the cultivation of high-quality A. argyi.
艾蒿是蒿属的一种草本植物，其幼叶和成熟叶分别用作食物和药物。腺毛（GTs）分布在艾蒿的叶表面，通常被认为是类黄酮生物合成和积累的位置。然而，艾蒿中类黄酮生物合成和积累的机制尚不清楚。在这项研究中，筛选和评估了参与该物种类黄酮生物合成和毛状体发育的核心调节基因，并揭示了艾蒿中关键类黄酮的生物合成途径。使用加权基因共表达网络分析筛选 AaMYB1 和 AaYABBY1，然后将这两个基因分别遗传转化到烟草（Nicotiana tabacum L. cv. K326）中。同时，将 AaYABBY1 遗传转化到拟南芥中。过表达 AaMYB1 和 AaYABBY1 的烟草植株中总黄酮和芦丁含量增加，参与黄酮合成途径的基因，如 PAL、FLS 和 F3H 的表达水平在过表达这些基因的植物中显著上调。这些结果表明 AaMYB1 和 AaYABBY1 促进了烟草中类黄酮的生物合成。此外，与野生型相比，过表达 AaYABBY1 的烟草和拟南芥植株的毛状体密度显著增加。这些结果证实 AaYABBY1 可能参与调控艾蒿毛状体的形成。这表明了参与其中的潜在基因，并为基于“发育-代谢”互作网络和高质量艾蒿的栽培，提供了毛状体细胞工厂发育的新见解。
