Cui Zhan-Hu, Li Shu-Jiao, Man Yong-Hong, Li Ming-Jie, Gu Li, Zhang Zhong-Yi, Li Meng-Zhi, Huang Xian-Zhang, Wang Yu-Qing
Institute of Nanyang Geoherbs (Artemisia argyi), Henan Key Laboratory of Traditional Chinese Medicine for Effective Substances and Quality Control, Nanyang Medical College Nanyang 473061, China Fujian Agriculture and Forestry University Fuzhou 350002, China.
Institute of Nanyang Geoherbs (Artemisia argyi), Henan Key Laboratory of Traditional Chinese Medicine for Effective Substances and Quality Control, Nanyang Medical College Nanyang 473061, China.
Zhongguo Zhong Yao Za Zhi. 2024 Aug;49(16):4407-4419. doi: 10.19540/j.cnki.cjcmm.20240415.103.
The MYB(v-myb avian myeloblastosis viral oncogene homolog) family of transcription factors is the largest class of genes among higher plant transcription factors, which can be divided into four subfamilies, with the R2R3-MYB being the most common subfamily type. R2R3-MYB transcription factors are widely involved in the regulation of organ development and secondary metabolite biosynthesis in plants. To investigate the role of R2R3-MYB family transcription factors in the synthesis of flavonoids and glandular trichome development in Artemisia argyi, this study screened and identified 92 R2R3-MYB transcription factors based on the whole genome data of A. argyi, and predicted their potential functions based on bioinformatics. The results showed that the amino acid lengths of the 92 transcription factors ranged from 168 to 547 aa, with relative molecular weights ranging from 19. 6 to 60. 5 kDa, all of which were hydrophilic proteins. Subcellular localization analysis showed that 89 AaMYB proteins were located in the nucleus, while three proteins were simultaneously located in the nucleus and cytoplasm. According to the classification of Arabidopsis R2R3-MYB family, the 92 A. argyi R2R3-MYB proteins were divided into 26 subfamilies, with similar gene structures within the same subfamily.Cis-acting element prediction results showed that light-responsive elements, methyl jasmonate elements, and abscisic acid elements were widely distributed in the promoter regions of R2R3-MYB genes. Transcriptome expression analysis results showed that the expression of AaMYB60, AaMYB63, and AaMYB86 in leaves was higher than that in stems and roots, indicating that these three transcription factors mainly function in leaves. Additionally, five candidate R2R3-MYB transcription factors involved in A. argyi flavonoid biosynthesis or glandular trichome development were selected through phylogenetic analysis. This study provides important genetic resources for the breeding of superior varieties and germplasm innovation of A. argyi in the future.
MYB（v-myb禽成髓细胞瘤病毒癌基因同源物）转录因子家族是高等植物转录因子中最大的一类基因，可分为四个亚家族，其中R2R3-MYB是最常见的亚家族类型。R2R3-MYB转录因子广泛参与植物器官发育和次生代谢物生物合成的调控。为了研究R2R3-MYB家族转录因子在艾草黄酮类化合物合成和腺毛发育中的作用，本研究基于艾草全基因组数据筛选并鉴定了92个R2R3-MYB转录因子，并通过生物信息学预测了它们的潜在功能。结果表明，92个转录因子的氨基酸长度在168至547个氨基酸之间，相对分子量在19.6至60.5 kDa之间，均为亲水性蛋白。亚细胞定位分析表明，89个AaMYB蛋白位于细胞核中，而3个蛋白同时位于细胞核和细胞质中。根据拟南芥R2R3-MYB家族的分类，92个艾草R2R3-MYB蛋白被分为26个亚家族，同一亚家族内基因结构相似。顺式作用元件预测结果表明，光响应元件、茉莉酸甲酯元件和脱落酸元件广泛分布于R2R3-MYB基因的启动子区域。转录组表达分析结果表明，AaMYB60、AaMYB63和AaMYB86在叶中的表达高于茎和根，表明这三个转录因子主要在叶中发挥作用。此外，通过系统发育分析筛选出5个参与艾草黄酮类化合物生物合成或腺毛发育的候选R2R3-MYB转录因子。本研究为今后艾草优良品种选育和种质创新提供了重要的遗传资源。
