Boronin A M, Filonov A E, Erova T E
Antibiot Med Biotekhnol. 1985 Jan;30(1):22-7.
Competition of the plasmid-containing strain C600 (RP4) (pBS94) of E. coli and the plasmid-free strain C600 rifr of E. coli in chemostatic and pH-static continuous cultures resulted in displacement of the plasmid-free strain in spite of its higher specific growth rate. Conjugative transfer of RP4 and pBS94 plasmids into the cells of the plasmid-free strain was observed in the experiments. Competition of strains C600 (pBS94) (RP4 Tra-) and C600 rifr in the chemostatic culture in the absence of the plasmid conjugative transfer resulted in displacement of the plasmid-containing strain from the population, while in the pH-static culture, the initial ratio of the plasmid-containing and plasmid-free bacteria was preserved. The conjugative transfer of the plasmids lowered the number of the plasmid-free cells in the population and might be considered as a factor stabilizing the plasmids in the bacterial population of the continuous cultures.
在恒化和恒pH连续培养中，含质粒的大肠杆菌菌株C600 (RP4) (pBS94) 与无质粒的大肠杆菌菌株C600 rifr竞争，尽管无质粒菌株的比生长速率较高，但仍导致其被取代。实验中观察到RP4和pBS94质粒向无质粒菌株细胞的接合转移。在无质粒接合转移的情况下，菌株C600 (pBS94) (RP4 Tra-) 和C600 rifr在恒化培养中的竞争导致含质粒菌株从群体中被取代，而在恒pH培养中，含质粒和无质粒细菌的初始比例得以保持。质粒的接合转移降低了群体中无质粒细胞的数量，可被视为在连续培养细菌群体中稳定质粒的一个因素。
