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质粒RP4的一种天然突变体,它赋予噬菌体抗性并降低接合转移能力。

A natural mutant of plasmid RP4 that confers phage resistance and reduced conjugative transfer.

作者信息

Kornstein L B, Waters V L, Cooper R C

机构信息

Sanitary Engineering and Environmental Health Research Laboratory, University of California, Berkeley.

出版信息

FEMS Microbiol Lett. 1992 Mar 1;70(2):97-100. doi: 10.1016/0378-1097(92)90666-c.

Abstract

A natural isolate of RP4 (PRC#116) acquired from the Stanford University Plasmid Reference Center differed from the wild-type Incompatibility Group P plasmid in several respects. Cells of Escherichia coli harboring PRC#116 were resistant to the IncP pili-specific bacteriophage PRD1 and GU5, and transferred this plasmid at a lower efficiency than the wild-type RP4. Phage sensitivity was restored, and transfer considerably improved in PRC#116+ bacteria transformed with plasmid constructs containing the origin of transfer (oriT region) of RP4. Mutant RP4 plasmids equivalent to PRC#116 were selected at a high frequency from an RP4+ E. coli population infected with PRD1 indicating that this RP4 variant may be the product of a very common mutation of the wild-type plasmid.

摘要

从斯坦福大学质粒参考中心获得的RP4自然分离株(PRC#116)在几个方面与野生型不相容群P质粒不同。携带PRC#116的大肠杆菌细胞对IncP菌毛特异性噬菌体PRD1和GU5具有抗性,并且该质粒的转移效率低于野生型RP4。在用含有RP4转移起始点(oriT区域)的质粒构建体转化的PRC#116+细菌中,噬菌体敏感性得以恢复,转移效率也显著提高。从感染PRD1的RP4+大肠杆菌群体中高频筛选出与PRC#116等效的突变RP4质粒,这表明该RP4变体可能是野生型质粒非常常见的突变产物。

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