Ruskova M
Vet Med Nauki. 1979;16(1):28-32.
A 24-hour culture of Escherichia coli 0138:K81:H19 was used to isolate a soluble cytoplasmic fraction, an O antigen, and an unsolube cell ingredient. Their immunogenic action was followed up through subcutaneous vaccination of mice and the production of humoral antibodies in rabbits. It was found that the cytoplasmic ingredient was a slightly toxic protective antigen forming considerable immunity in mice and a comparatively high titer of antibodies in rabbit sera. The unsoluble cell ingredient and the O antigen proved comparatively toxic and did not produce favourable immune response. Discussed in the use of the cytoplasmic ingredient as a vaccine as well as the type of the immunoglobulins produced.
使用大肠杆菌0138:K81:H19的24小时培养物来分离可溶性细胞质部分、O抗原和不溶性细胞成分。通过对小鼠进行皮下接种疫苗以及在兔体内产生体液抗体来跟踪它们的免疫原性作用。发现细胞质成分是一种毒性稍弱的保护性抗原，在小鼠体内形成相当强的免疫力，在兔血清中产生相对较高滴度的抗体。不溶性细胞成分和O抗原被证明毒性相对较大，且未产生良好的免疫反应。文中还讨论了将细胞质成分用作疫苗的用途以及所产生的免疫球蛋白类型。
