Key Laboratory of Agricultural Microbiomics and Precision Application (MARA), Key Laboratory of Agricultural Microbiome (MARA), Guangdong Microbial Culture Collection Center (GDMCC), Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, PR China.
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Horticulture, South China Agricultural University, Guangzhou, 510642, PR China.
Int J Syst Evol Microbiol. 2024 Jun;74(6). doi: 10.1099/ijsem.0.006430.
A novel Gram-stain-negative strain, designated JM10B15, was isolated from pond water for collected from Jiangmen City, Guangdong province, south PR China. Cells of the strain were aerobic, rod-shaped, and motile by lateral flagella. JM10B15 could grow at 15-40 °C, pH 6.0-9.5, and in 0-3.0 % NaCl, with optimal growth at 25-35 °C, pH 7.5-8.5, and in 0 % NaCl, respectively. Furthermore, this strain grew well on Reasoner's 2A agar but not on nutrient broth agar or Luria-Bertani agar. JM10B15 was a denitrifying bacterium capable of removing nitrites and nitrates, and three key functional genes, , , and , were identified in its genome. The results of phylogenetic analyses based on the 16S rRNA gene and genome sequences indicated that JM10B15 belonged to the genus . JM10B15 showed the highest 16S rRNA sequence similarity to YJ-T1-11 (98.8 %), followed by IFAM 1031 (98.6 %) and HB-1 (98.1 %). The average nucleotide identity and digital DNA-DNA hybridization values between JM10B15 and the other type strains of genus were 78.1-82.1 % and 18.4-22.1 %, respectively. The major fatty acids of strain JM10B15 were summed feature 8 (C 6 and/or C 7) and C 7 11-methyl. In addition, the major respiratory quinone of this novel strain was Q-10, and the predominant polar lipids were phosphatidylglycerol, phosphatidylethanolamine, four unidentified phospholipids, three unidentified lipids, and an unidentified aminophospholipid. Results of analyses of the phylogenetic, genomic, physiological, and biochemical characteristics indicated that JM10B15 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JM10B15 (=GDMCC 1.4148=KCTC 8140).
一株新的革兰氏阴性菌株，命名为 JM10B15，是从中国广东省江门市采集的池塘水中分离出来的。该菌株的细胞呈需氧、杆状，通过侧鞭毛运动。JM10B15 可以在 15-40°C、pH 6.0-9.5 和 0-3.0%NaCl 下生长，最佳生长条件为 25-35°C、pH 7.5-8.5 和 0%NaCl。此外，该菌株在 Reasoner's 2A 琼脂上生长良好，但在营养肉汤琼脂或 Luria-Bertani 琼脂上不能生长。JM10B15 是一种能够去除亚硝酸盐和硝酸盐的反硝化细菌，并且在其基因组中鉴定出三个关键的功能基因、、和。基于 16S rRNA 基因和基因组序列的系统发育分析结果表明，JM10B15 属于属。JM10B15 与 YJ-T1-11（98.8%）的 16S rRNA 序列相似度最高，其次是 IFAM 1031（98.6%）和 HB-1（98.1%）。JM10B15 与属其他模式菌株的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 78.1-82.1%和 18.4-22.1%。菌株 JM10B15 的主要脂肪酸为总和特征 8（C6 和/或 C7）和 C7 11-甲基。此外，该新型菌株的主要呼吸醌为 Q-10，主要极性脂为磷脂酰甘油、磷脂酰乙醇胺、四种未鉴定的磷脂、三种未鉴定的脂类和一种未鉴定的氨基磷脂。基于系统发育、基因组、生理和生化特征的分析结果表明，JM10B15 代表属的一个新种，建议将其命名为 sp. nov.。该模式菌株是 JM10B15（=GDMCC 1.4148=KCTC 8140）。
