Department of Pediatrics III, Cystic Fibrosis Centre Innsbruck, Medical University of Innsbruck, Innsbruck, Austria.
Tiroler Gesundheitsfond, Amt der Tiroler Landesregierung, Innsbruck, Austria.
BMC Pulm Med. 2024 Jun 19;24(1):286. doi: 10.1186/s12890-024-03103-9.
Cystic fibrosis (CF) is a genetic multisystem disorder. Inflammatory processes, which presumably begin early in infancy, play a crucial role in the progression of the disease. The detection of inflammatory biomarkers, especially in the airways, has therefore gained increasing attention. Due to improved treatment options, patients with CF produce less sputum. Nasal lavage samples therefore represent a promising alternative to induced sputum or bronchoalveolar lavage specimens. However, methodology of cytokine measurements is not standardised and comparisons of results are therefore often difficult. The aim of this study was to identify suitable detection methods of cytokines in nasal lavage samples by comparison of two different assays.
Nasal lavage samples were obtained from the same patient at the same time by trained respiratory physiotherapists using a disposable syringe and 10 ml of 0.9% sodium chloride per nostril during outpatient visits. The cytokines IL-17 A, IL-2, IL-6 and IL-10 were measured using two different assays (BD™ and Milliplex®), which have already been applied in sputum and nasal lavage samples, despite different lower detection limits.
22 participants were included in the study. In 95.5% of measurements, values were below the limit of detection with respect to the BD™ assay. Only IL-6 could be detected in approximately half of the patients. Individual cytokine levels were considerably higher when measured with Milliplex®, which is also reflected in a statistically significant manner (p = < 0.01).
The right choice of analysis method is crucial for measuring inflammatory markers in nasal lavage samples. Compared to the literature, Milliplex® showed higher detection rates and similar concentrations to other studies.
Ethics approval was obtained from the ethics committee at Medical University of Innsbruck (EK Nr: 1055/2022).
囊性纤维化（CF）是一种遗传多系统疾病。炎症过程，据推测在婴儿早期就开始了，在疾病的进展中起着至关重要的作用。因此，炎症生物标志物的检测，特别是在气道中，越来越受到关注。由于治疗选择的改善，CF 患者产生的痰液减少。因此，鼻洗液样本代表了诱导痰或支气管肺泡灌洗液标本的有前途的替代物。然而，细胞因子测量的方法尚未标准化，因此结果的比较通常很困难。本研究的目的是通过比较两种不同的检测方法来确定鼻洗液样本中细胞因子的合适检测方法。
在门诊就诊时，由经过培训的呼吸理疗师使用一次性注射器和每侧鼻孔 10 毫升 0.9%氯化钠溶液从同一患者的同一时间获得鼻洗液样本。使用两种不同的检测方法（BD ™和 Milliplex ®）测量细胞因子 IL-17 A、IL-2、IL-6 和 IL-10，这两种方法已经在痰和鼻洗液样本中得到应用，尽管检测下限不同。
本研究共纳入 22 名参与者。在 95.5%的测量中，BD ™检测方法的检测值低于检测下限。只有大约一半的患者可以检测到 IL-6。当使用 Milliplex ®测量时，个别细胞因子水平要高得多，这也以统计学上显著的方式反映出来（p < 0.01）。
分析方法的正确选择对于测量鼻洗液样本中的炎症标志物至关重要。与文献相比，Milliplex ®显示出更高的检测率和与其他研究相似的浓度。
因斯布鲁克医科大学伦理委员会（EK Nr：1055/2022）批准了该研究。
