囊性纤维化患者鼻洗液细胞因子的检测：两种不同细胞因子检测方法的比较。

Detection of cytokines in nasal lavage samples of patients with cystic fibrosis: comparison of two different cytokine detection assays.

机构信息

Department of Pediatrics III, Cystic Fibrosis Centre Innsbruck, Medical University of Innsbruck, Innsbruck, Austria.

Tiroler Gesundheitsfond, Amt der Tiroler Landesregierung, Innsbruck, Austria.

出版信息

BMC Pulm Med. 2024 Jun 19;24(1):286. doi: 10.1186/s12890-024-03103-9.

DOI:10.1186/s12890-024-03103-9

PMID:38890643

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11186286/

Abstract

BACKGROUND

Cystic fibrosis (CF) is a genetic multisystem disorder. Inflammatory processes, which presumably begin early in infancy, play a crucial role in the progression of the disease. The detection of inflammatory biomarkers, especially in the airways, has therefore gained increasing attention. Due to improved treatment options, patients with CF produce less sputum. Nasal lavage samples therefore represent a promising alternative to induced sputum or bronchoalveolar lavage specimens. However, methodology of cytokine measurements is not standardised and comparisons of results are therefore often difficult. The aim of this study was to identify suitable detection methods of cytokines in nasal lavage samples by comparison of two different assays.

METHODS

Nasal lavage samples were obtained from the same patient at the same time by trained respiratory physiotherapists using a disposable syringe and 10 ml of 0.9% sodium chloride per nostril during outpatient visits. The cytokines IL-17 A, IL-2, IL-6 and IL-10 were measured using two different assays (BD™ and Milliplex®), which have already been applied in sputum and nasal lavage samples, despite different lower detection limits.

RESULTS

22 participants were included in the study. In 95.5% of measurements, values were below the limit of detection with respect to the BD™ assay. Only IL-6 could be detected in approximately half of the patients. Individual cytokine levels were considerably higher when measured with Milliplex®, which is also reflected in a statistically significant manner (p = < 0.01).

CONCLUSION

The right choice of analysis method is crucial for measuring inflammatory markers in nasal lavage samples. Compared to the literature, Milliplex® showed higher detection rates and similar concentrations to other studies.

TRIAL REGISTRATION

Ethics approval was obtained from the ethics committee at Medical University of Innsbruck (EK Nr: 1055/2022).

摘要

背景

囊性纤维化（CF）是一种遗传多系统疾病。炎症过程，据推测在婴儿早期就开始了，在疾病的进展中起着至关重要的作用。因此，炎症生物标志物的检测，特别是在气道中，越来越受到关注。由于治疗选择的改善，CF 患者产生的痰液减少。因此，鼻洗液样本代表了诱导痰或支气管肺泡灌洗液标本的有前途的替代物。然而，细胞因子测量的方法尚未标准化，因此结果的比较通常很困难。本研究的目的是通过比较两种不同的检测方法来确定鼻洗液样本中细胞因子的合适检测方法。

方法

在门诊就诊时，由经过培训的呼吸理疗师使用一次性注射器和每侧鼻孔 10 毫升 0.9%氯化钠溶液从同一患者的同一时间获得鼻洗液样本。使用两种不同的检测方法（BD ™和 Milliplex ®）测量细胞因子 IL-17 A、IL-2、IL-6 和 IL-10，这两种方法已经在痰和鼻洗液样本中得到应用，尽管检测下限不同。