Cui Pengbo, Liang Jianqin, Cheng Tianyu, Zhang Jianyou
Zhejiang Key Laboratory of Green, Low-Carbon and Efficient Development of Marine Fishery Resources, College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
Foods. 2024 May 25;13(11):1652. doi: 10.3390/foods13111652.
The purpose of this study was to prepare mackerel peptides (MPs) with calcium-binding capacity through an enzyme method and to investigate the potential role they play in improving the bioavailability of calcium in vitro. The calcium-binding capacity, degree of hydrolysis (DH), molecular weight (MW), and charge distribution changes with the enzymolysis time of MPs were measured. The structural characterization of mackerel peptide-calcium (MP-calcium) complexes was performed using spectroscopy and morphology analysis. The results showed that the maximum calcium-binding capacity of the obtained MPs was 120.95 mg/g when alcalase was used for 3 h, with a DH of 15.45%. Moreover, with an increase in hydrolysis time, the MW of the MPs decreased, and the negative charge increased. The carboxyl and amino groups in aspartic (Asp) and glutamate (Glu) of the MPs may act as calcium-binding sites, which are further assembled into compact nanoscale spherical complexes with calcium ions through intermolecular interactions. Furthermore, even under the influence of oxalic acid, MP-calcium complexes maintained a certain solubility. This study provides a basis for developing new calcium supplements and efficiently utilizing the mackerel protein resource.
本研究的目的是通过酶法制备具有钙结合能力的鲭鱼肽（MPs），并研究它们在体外提高钙生物利用度方面的潜在作用。测定了MPs的钙结合能力、水解度（DH）、分子量（MW）以及随着酶解时间电荷分布的变化。使用光谱学和形态分析对鲭鱼肽 - 钙（MP - 钙）复合物进行结构表征。结果表明，当使用碱性蛋白酶3小时时，所获得的MPs的最大钙结合能力为120.95 mg/g，水解度为15.45%。此外，随着水解时间的增加，MPs的分子量降低，负电荷增加。MPs中天冬氨酸（Asp）和谷氨酸（Glu）中的羧基和氨基可能作为钙结合位点，通过分子间相互作用进一步与钙离子组装成紧密的纳米级球形复合物。此外，即使在草酸的影响下，MP - 钙复合物仍保持一定的溶解度。本研究为开发新型钙补充剂和有效利用鲭鱼蛋白资源提供了依据。
