LSRB-DRDO Project, JSS College of Arts, Commerce and Science, Mysuru, India.
Postgraduate Department of Biochemistry, JSS College of Arts, Commerce and Science, Mysuru, India.
J Food Biochem. 2022 Jul;46(7):e14102. doi: 10.1111/jfbc.14102. Epub 2022 Feb 12.
In the present investigation, a metal-chelating bioactive peptide was derived from Citrullus lanatus seed milk fermented with Lactococcus lactis. The cationic fermented milk peptide (FMP) thus obtained was purified using the HiTrap-chelating column followed by rpHPLC. The FMP possessed the ability to chelate multiple divalent cations like Cu , Ca , and Fe with 86.81%, 61.04%, and 24.32% of chelation respectively and further it exhibited 78.03% of DPPH free radical scavenging activity. Interaction of FMP with metal ions was assessed by change in the absorption spectra and was analyzed by ultraviolet-visible and fluorescence spectroscopy. The FMP-metal complexes were found stable at simulated gastric conditions. In vitro analysis using intestinal Caco-2 cell lines revealed that there was an increase in metal bioavailability in the presence of the FMP and was least influenced by the addition of a dietary inhibitor, phytic acid. By LC-MS analysis the molecular mass of FMP was found to be 11.6 kD and it contains oxygen-rich and nitrogen-rich amino acids that favor the metal chelation. In our study, we have found that the fermented C. lanatus seed milk can serve as a potential functional food with bioenhancer peptides that increase metal bioavailability and enhance human health. PRACTICAL APPLICATIONS: Chelated metals are preferred over non-chelated ones by most nutritionists for their better absorption rate. Chelation protects the minerals from the digestive process and increases their bioavailability. Fermentation with lactic acid bacteria produces bioactive peptides with metal-chelating and antioxidant ability which provides additional health benefits beyond supplying basic nutrients. Lactococcus lactis fermented milk acts as a probiotic product with bioenhancer peptide that increases mineral bioavailability. Consumption of metals in chelated form can reduce excess intake of metal. Fermented watermelon seed milk can be a promising probiotic drink rich in bioenhancer peptides and can enhance the bioavailability of divalent cations of a high therapeutic index.
在本研究中，从发酵乳球菌发酵的西瓜种子乳中衍生出一种金属螯合生物活性肽。所得的阳离子发酵乳肽（FMP）通过 HiTrap-螯合柱进行纯化，然后通过反相高效液相色谱法（rpHPLC）进行纯化。FMP 具有螯合多种二价阳离子的能力，分别为 Cu、Ca 和 Fe 的 86.81%、61.04%和 24.32%，进一步表现出 78.03%的 DPPH 自由基清除活性。通过吸收光谱的变化评估 FMP 与金属离子的相互作用，并通过紫外-可见和荧光光谱分析进行分析。FMP-金属复合物在模拟胃条件下稳定。使用肠 Caco-2 细胞系进行的体外分析表明，在 FMP 的存在下，金属的生物利用度增加，并且受膳食抑制剂植酸的添加影响最小。通过 LC-MS 分析，FMP 的分子量被发现为 11.6 kD，它含有富含氧和氮的氨基酸，有利于金属螯合。在我们的研究中，我们发现发酵的西瓜种子乳可以作为一种潜在的功能性食品，具有增加金属生物利用度和增强人类健康的生物增强肽。 实际应用：大多数营养师更喜欢螯合金属而不是非螯合金属，因为它们的吸收率更高。螯合作用可保护矿物质免受消化过程的影响，并提高其生物利用度。与乳酸菌发酵产生具有金属螯合和抗氧化能力的生物活性肽，除了提供基本营养外，还提供额外的健康益处。发酵乳球菌发酵乳作为一种含有生物增强肽的益生菌产品，可以增加矿物质的生物利用度。以螯合形式摄入金属可以减少金属过量摄入。发酵西瓜种子乳可以成为一种很有前途的富含生物增强肽的益生菌饮料，可以提高高治疗指数二价阳离子的生物利用度。
