The Utilization by spp. of a Purified Polysaccharide from Fuzhuan Brick Tea.

In the present study, four species that could degrade Fuzhuan brick tea polysaccharide-3 (FBTPS-3) were isolated from human feces and identified to be , . The four species showed growth on FBTPS-3 as the carbon source, and showed the best capability for utilizing FBTPS-3 among the four species since could utilize more FBTPS-3 during 24 h fermentation. Moreover, the four species could metabolize FBTPS-3 and promote the production of acetic, propionic and isovaleric acids. Transcriptome analysis of revealed that 602 genes were up-regulated by FBTPS-3, including two carbohydrate-active enzyme clusters and four polysaccharide utilization loci (PULs). The PUL 1 contained GH28 family that could hydrolyze rhamnogalacturonan and other pectic substrates, which was in line with our previous work that rhamnose and galacturonic acid were the main component monosaccharides of FBTPS-3. Collectively, the results suggested that FBTPS-3 could be utilized by spp., and it might be developed as a promising prebiotic targeting Bacteroidetes in intestinal environment.