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用于普氏粪杆菌的多功能基因工具箱可用于研究多糖利用系统。

A versatile genetic toolbox for Prevotella copri enables studying polysaccharide utilization systems.

作者信息

Li Jing, Gálvez Eric J C, Amend Lena, Almási Éva, Iljazovic Aida, Lesker Till R, Bielecka Agata A, Schorr Eva-Magdalena, Strowig Till

机构信息

Department of Microbial Immune Regulation, Helmholtz Centre for Infection Research, Braunschweig, Germany.

Hannover Medical School, Hannover, Germany.

出版信息

EMBO J. 2021 Dec 1;40(23):e108287. doi: 10.15252/embj.2021108287. Epub 2021 Oct 21.

DOI:10.15252/embj.2021108287
PMID:34676563
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8634118/
Abstract

Prevotella copri is a prevalent inhabitant of the human gut and has been associated with plant-rich diet consumption and diverse health states. The underlying genetic basis of these associations remains enigmatic due to the lack of genetic tools. Here, we developed a novel versatile genetic toolbox for rapid and efficient genetic insertion and allelic exchange applicable to P. copri strains from multiple clades. Enabled by the genetic platform, we systematically investigated the specificity of polysaccharide utilization loci (PULs) and identified four highly conserved PULs for utilizing arabinan, pectic galactan, arabinoxylan, and inulin, respectively. Further genetic and functional analysis of arabinan utilization systems illustrate that P. copri has evolved two distinct types of arabinan-processing PULs (PUL ) and that the type-II PUL is significantly enriched in individuals consuming a vegan diet compared to other diets. In summary, this genetic toolbox will enable functional genetic studies for P. copri in future.

摘要

普氏粪杆菌是人类肠道中的常见居民，与富含植物的饮食摄入以及多种健康状况有关。由于缺乏遗传工具，这些关联背后的遗传基础仍然不明。在此，我们开发了一种新型通用遗传工具箱，用于快速高效的基因插入和等位基因交换，适用于多个进化枝的普氏粪杆菌菌株。借助该遗传平台，我们系统地研究了多糖利用位点（PULs）的特异性，并分别鉴定出四个高度保守的PULs，用于利用阿拉伯聚糖、果胶半乳聚糖、阿拉伯木聚糖和菊粉。对阿拉伯聚糖利用系统的进一步遗传和功能分析表明，普氏粪杆菌进化出了两种不同类型的阿拉伯聚糖加工PULs（PUL），与其他饮食相比，II型PUL在食用纯素饮食的个体中显著富集。总之，这个遗传工具箱将为未来普氏粪杆菌的功能遗传学研究提供支持。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/92ed437bf858/EMBJ-40-e108287-g011.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/cbbb84f7a9b7/EMBJ-40-e108287-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/cb413f3ec3e5/EMBJ-40-e108287-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/35eacf1adc69/EMBJ-40-e108287-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/e26456f62499/EMBJ-40-e108287-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/92ed437bf858/EMBJ-40-e108287-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/7d881f9e8638/EMBJ-40-e108287-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/a1e37d214649/EMBJ-40-e108287-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/e1ede149d662/EMBJ-40-e108287-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/97e1bf179c15/EMBJ-40-e108287-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/cbbb84f7a9b7/EMBJ-40-e108287-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/cb413f3ec3e5/EMBJ-40-e108287-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/35eacf1adc69/EMBJ-40-e108287-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/aced7245b878/EMBJ-40-e108287-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/7893885d5c28/EMBJ-40-e108287-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/e26456f62499/EMBJ-40-e108287-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/aa9a/8634118/92ed437bf858/EMBJ-40-e108287-g011.jpg

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