Genome-Wide Identification, Characterization and Expression Analysis of the JAZ Gene Family in Resistance to Gray Leaf Spots in Tomato.

The plant disease resistance system involves a very complex regulatory network in which jasmonates play a key role in response to external biotic or abiotic stresses. As inhibitors of the jasmonic acid (JA) signaling pathway, JASMONATE ZIM domain (JAZ) proteins have been identified in many plant species, and their functions are gradually being clarified. In this study, 26 genes were identified in tomato. The physical and chemical properties, predicted subcellular localization, gene structure, cis-acting elements, and interspecies collinearity of 26 genes were subsequently analyzed. RNA-seq data combined with qRT-PCR analysis data showed that the expression of most genes were induced in response to , methyl jasmonate (MeJA) and salicylic acid (SA). Tobacco rattle virus RNA2-based VIGS vector (TRV2)- plants were more resistant to tomato gray leaf spots than TRV2-00 plants. Therefore, we speculated that played a negative regulatory role in tomato resistance to gray leaf spots. Based on combining the results of previous studies and those of our experiments, we speculated that might be closely related to JA and SA hormone regulation. interacted with , , , and other resistance-related genes to form a regulatory network, and these genes played an important role in the regulation of tomato gray leaf spots. The subcellular localization results showed that the gene was located in the nucleus. Overall, this study is the first to identify and analyze family genes in tomato via bioinformatics approaches, clarifying the regulatory role of genes in tomato resistance to gray leaf spots and providing new ideas for improving plant disease resistance.