Yi Songheng, Cai Qihang, Yang Yanbo, Shen Hongquan, Sun Zhenghai, Li Liping
College of Landscape and Horticulture, Southwest Forestry University, Kunming 650224, China.
College of Geography and Ecotourism, Southwest Forestry University, Kunming 650224, China.
Plants (Basel). 2024 Jun 6;13(11):1570. doi: 10.3390/plants13111570.
The MYB transcription factors (TFs) have substantial functions in anthocyanin synthesis as well as being widely associated with plant responses to various adversities. In the present investigation, we found an unreported MYB TF from (a wild relative of eggplant) and named it in reference to its homologous gene. Bioinformatics analysis demonstrated that the open reading frame of was 825 bp in length, encoding 275 amino acids, with a typical R2R3-MYB gene structure, and predicted subcellular localization in the nucleus. Analysis of the tissue-specific expression pattern through qRT-PCR showed that the was expressed at a high level in young stems as well as leaves of . Transgenic and tobacco plants overexpressing pertinent to the control of the 35S promoter exhibited a distinct purple color trait, suggesting a significant change in their anthocyanin content. Furthermore, we obtained three tobacco transgenic lines with significant differences in anthocyanin accumulation and analyzed the differences in anthocyanin content by LC-MS/MS. The findings demonstrated that overexpression of caused tobacco to have considerably raised levels of several anthocyanin components, with the most significant increases in delphinidin-like anthocyanins and cyanidin-like anthocyanins. The qRT-PCR findings revealed significant differences in the expression levels of structural genes for anthocyanin synthesis among various transgenic lines. In summary, this study demonstrated that the gene has a substantial impact on anthocyanin synthesis, and overexpression of the gene leads to significant modifications to the expression levels of a variety of anthocyanin-synthesizing genes, which leads to complex changes in anthocyanin content and affects plant phenotypes. This present research offers the molecular foundation for the research of the mechanism of anthocyanin formation within plants, as well as providing some reference for the improvement of traits in solanum crops.
MYB转录因子（TFs）在花青素合成中具有重要功能，并且广泛参与植物对各种逆境的响应。在本研究中，我们从（茄子的野生近缘种）中发现了一个未报道的MYB转录因子，并根据其同源基因将其命名为。生物信息学分析表明，的开放阅读框长度为825 bp，编码275个氨基酸，具有典型的R2R3-MYB基因结构，并预测其亚细胞定位在细胞核中。通过qRT-PCR分析组织特异性表达模式表明，在的幼茎和叶片中高表达。过表达与35S启动子相关的的转基因和烟草植株表现出明显的紫色性状，表明其花青素含量发生了显著变化。此外，我们获得了三个花青素积累有显著差异的烟草转基因株系，并通过LC-MS/MS分析了花青素含量的差异。结果表明，的过表达使烟草中几种花青素成分的含量大幅提高，其中飞燕草素类花青素和矢车菊素类花青素增加最为显著。qRT-PCR结果显示，不同转基因株系中花青素合成结构基因的表达水平存在显著差异。总之，本研究表明基因对花青素合成有重要影响，基因的过表达导致多种花青素合成基因的表达水平发生显著改变，进而导致花青素含量的复杂变化并影响植物表型。本研究为植物花青素形成机制的研究提供了分子基础，也为茄科作物性状改良提供了一定参考。
