Active Botanicals Research (ABR), 36040 Brendola, Italy.
Department of Biology, University of Padua, 35131 Padua, Italy.
Int J Mol Sci. 2023 Sep 5;24(18):13711. doi: 10.3390/ijms241813711.
Plant cell cultures have emerged as a promising tool for producing active molecules due to their numerous advantages over traditional agricultural methods. Flavonols, and anthocyanin pigments in particular, together with other phenolic compounds such as chlorogenic acid, are known for their beneficial health properties, mainly due to their antioxidant, antimicrobial, and anti-inflammatory activities. The synthesis of these molecules is finely regulated in plant cells and controlled at the transcriptional level by specific MYB and bHLH transcription factors that coordinate the transcription of structural biosynthetic genes. The co-expression of peach and in tobacco was used to develop tobacco cell lines showing high expression of both the peach transgenes and the native flavonol structural genes. These cell lines were further selected for fast growth. High production levels of chlorogenic acid, anthocyanins (mainly cyanidin 3-rutinoside), and other phenolics were also achieved in pre-industrial scale-up trials. A single-column-based purification protocol was developed to produce a lyophile called ANT-CA, which was stable over time, showed beneficial effects on cell viability, and had antioxidant, anti-inflammatory, antibacterial, and wound-healing activities. This lyophile could be a valuable ingredient for food or cosmetic applications.
植物细胞培养已成为生产活性分子的有前途的工具，因为它们具有许多优于传统农业方法的优势。类黄酮，特别是花色苷和其他酚类化合物，如绿原酸，因其具有有益的健康特性而闻名，主要是由于其抗氧化、抗菌和抗炎活性。这些分子在植物细胞中的合成受到精细调节，并通过特定的 MYB 和 bHLH 转录因子在转录水平上受到控制，这些转录因子协调结构生物合成基因的转录。桃和在烟草中的共表达被用于开发表现出桃转基因和天然类黄酮结构基因高表达的烟草细胞系。这些细胞系进一步被选择用于快速生长。在预工业化的放大试验中还实现了绿原酸、花色苷（主要是飞燕草素 3-芸香糖苷）和其他酚类物质的高产量。开发了一种基于单柱的纯化方案来生产一种名为 ANT-CA 的冻干物，该冻干物具有时间稳定性，对细胞活力有有益的影响，并且具有抗氧化、抗炎、抗菌和伤口愈合活性。这种冻干物可以成为食品或化妆品应用的有价值的成分。
