Center for Research and Engineering in Space Technologies, Universit libre de Bruxelles, 1050 Brussels, Belgium.
GIGA-In Silico Medicine, University of Liège, 4000 Liège, Belgium.
Int J Mol Sci. 2024 May 21;25(11):5600. doi: 10.3390/ijms25115600.
In the process of tissue engineering, several types of stresses can influence the outcome of tissue regeneration. This outcome can be understood by designing hydrogels that mimic this process and studying how such hydrogel scaffolds and cells behave under a set of stresses. Here, a hydrogel formulation is proposed to create biomimetic scaffolds suitable for fibroblast cell culture. Subsequently, we examine the impact of external stresses on fibroblast cells cultured on both solid and porous hydrogels. These stresses included mechanical tension and altered-gravity conditions experienced during the 83rd parabolic flight campaign conducted by the European Space Agency. This study shows distinct cellular responses characterized by cell aggregation and redistribution in regions of intensified stress concentration. This paper presents a new biomimetic hydrogel that fulfills tissue-engineering requirements in terms of biocompatibility and mechanical stability. Moreover, it contributes to our comprehension of cellular biomechanics under diverse gravitational conditions, shedding light on the dynamic cellular adaptations versus varying stress environments.
在组织工程学的过程中，有几种类型的应力会影响组织再生的结果。通过设计模拟该过程的水凝胶，并研究在一组应力下这种水凝胶支架和细胞的行为，可以理解这种结果。在这里，提出了一种水凝胶配方，以创建适合成纤维细胞培养的仿生支架。随后，我们研究了外部应力对培养在固体和多孔水凝胶上的成纤维细胞的影响。这些应力包括在欧洲航天局进行的第 83 次抛物线飞行任务中经历的机械张力和变重力条件。这项研究显示了明显的细胞反应，其特征是在应力集中区域发生细胞聚集和重新分布。本文提出了一种新的仿生水凝胶，它在生物相容性和机械稳定性方面满足组织工程的要求。此外，它有助于我们理解在不同重力条件下的细胞生物力学，揭示了在不同的应力环境下细胞的动态适应。
