National Key Laboratory of Smart Farm Technology and System, Key Laboratory of Soybean Biology in Chinese Ministry of Education, College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
Heilongjiang Green Food Science Research Institute, Harbin 150000, China.
Int J Mol Sci. 2024 May 21;25(11):5614. doi: 10.3390/ijms25115614.
Soybean, a major source of oil and protein, has seen an annual increase in consumption when used in soybean-derived products and the broadening of its cultivation range. The demand for soybean necessitates a better understanding of the regulatory networks driving storage protein accumulation and oil biosynthesis to broaden its positive impact on human health. In this study, we selected a chromosome segment substitution line (CSSL) with high protein and low oil contents to investigate the underlying effect of donor introgression on seed storage through multi-omics analysis. In total, 1479 differentially expressed genes (DEGs), 82 differentially expressed proteins (DEPs), and 34 differentially expressed metabolites (DEMs) were identified in the CSSL compared to the recurrent parent. Based on Gene Ontology (GO) term analysis and the Kyoto Encyclopedia of Genes and Genomes enrichment (KEGG), integrated analysis indicated that 31 DEGs, 24 DEPs, and 13 DEMs were related to seed storage functionality. Integrated analysis further showed a significant decrease in the contents of the seed storage lipids LysoPG 16:0 and LysoPC 18:4 as well as an increase in the contents of organic acids such as L-malic acid. Taken together, these results offer new insights into the molecular mechanisms of seed storage and provide guidance for the molecular breeding of new favorable soybean varieties.
大豆是油和蛋白质的主要来源，其衍生产品的消费逐年增加，种植范围也在扩大。对大豆的需求要求更好地了解调控网络，以促进其在人类健康方面的积极影响，这些网络驱动着贮藏蛋白的积累和油脂的生物合成。在本研究中，我们选择了高蛋白、低油的染色体片段代换系（CSSL），通过多组学分析来研究供体渐渗对种子贮藏的潜在影响。与轮回亲本相比，CSSL 中鉴定出了 1479 个差异表达基因（DEGs）、82 个差异表达蛋白（DEPs）和 34 个差异表达代谢物（DEMs）。基于基因本体论（GO）术语分析和京都基因与基因组百科全书（KEGG）富集分析，综合分析表明，31 个 DEGs、24 个 DEPs 和 13 个 DEMs 与种子贮藏功能有关。综合分析还表明，种子贮藏脂质 LysoPG 16:0 和 LysoPC 18:4 的含量显著降低，而有机酸如 L-苹果酸的含量增加。综上所述，这些结果为种子贮藏的分子机制提供了新的见解，并为新的有利大豆品种的分子育种提供了指导。
