College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
Lab of Functional Genomics and Bioinformatics, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Int J Mol Sci. 2022 Jun 20;23(12):6869. doi: 10.3390/ijms23126869.
Soybean is an important crop grown worldwide, and drought stress seriously affects the yield and quality of soybean. Therefore, it is necessary to elucidate the molecular mechanisms underlying soybean resistance to drought stress. In this study, RNA-seq technology and ultra-performance liquid chromatography-tandem mass spectrometry were used to analyze the transcriptome and metabolome changes in soybean leaves at the seedling stage under drought stress. The results showed that there were 4790 and 3483 DEGs (differentially expressed genes) and 156 and 124 DAMs (differentially expressed metabolites), respectively, in the HN65CK vs. HN65S0 and HN44CK vs. HN44S0 comparison groups. Comprehensive analysis of transcriptomic and metabolomic data reveals metabolic regulation of seedling soybean in response to drought stress. Some candidate genes such as , , and , , and showed different expression trends between the two cultivars, which may cause differences in drought resistance. Secondly, a large number of flavonoids were identified, and the expression of Monohydroxy-trimethoxyflavone-O-(6″-malonyl)glucoside was upregulated between the two varieties. Finally, several key candidate genes and metabolites involved in isoflavone biosynthesis and the TCA cycle were identified, suggesting that these metabolic pathways play important roles in soybean response to drought. Our study deepens the understanding of soybean drought resistance mechanisms and provides references for soybean drought resistance breeding.
大豆是世界范围内广泛种植的重要作物，干旱胁迫严重影响大豆的产量和品质。因此，阐明大豆抗旱的分子机制是十分必要的。本研究采用 RNA-seq 技术和超高效液相色谱-串联质谱联用技术，分析了干旱胁迫下大豆苗期叶片的转录组和代谢组变化。结果表明，在 HN65CK 与 HN65S0 及 HN44CK 与 HN44S0 比较组中，分别有 4790 个和 3483 个 DEGs（差异表达基因）和 156 个和 124 个 DAMs（差异表达代谢物）。综合转录组和代谢组数据分析揭示了幼苗大豆对干旱胁迫的代谢调控。一些候选基因如 、 、 、 、 和 ，在两个品种间表现出不同的表达趋势，这可能导致其抗旱性的差异。其次，鉴定出大量的类黄酮，两种品种之间 Monohydroxy-trimethoxyflavone-O-(6″-malonyl)glucoside 的表达上调。最后，鉴定出几个涉及异黄酮生物合成和 TCA 循环的关键候选基因和代谢物，表明这些代谢途径在大豆响应干旱胁迫中发挥着重要作用。本研究加深了对大豆抗旱机制的理解，为大豆抗旱育种提供了参考。
