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揭示隐藏基因:次级代谢产物生物合成相关基因的新见解。

Revealing Hidden Genes in : New Insights into Genes Involved in the Biosynthesis of Secondary Metabolites.

机构信息

Laboratorio de Microbiología, Departamento de Biomedicina, Biotecnología y Salud Pública, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain.

Departamento de Química Orgánica, Facultad de Ciencias, Campus Universitario Río San Pedro s/n, Torre sur, 4ª planta, Universidad de Cádiz, 11510 Puerto Real, Cádiz, Spain.

出版信息

Int J Mol Sci. 2024 May 28;25(11):5900. doi: 10.3390/ijms25115900.

Abstract

Utilizing bioinformatics tools, this study expands our understanding of secondary metabolism in , identifying novel genes within polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), sesquiterpene cyclase (STC), diterpene cyclase (DTC), and dimethylallyltryptophan synthase (DMATS) families. These findings enrich the genetic framework associated with 's pathogenicity and ecological adaptation, offering insights into uncharted metabolic pathways. Significantly, the discovery of previously unannotated genes provides new molecular targets for developing targeted antifungal strategies, promising to enhance crop protection and advance our understanding of fungal biochemistry. This research not only broadens the scope of known secondary metabolites but also opens avenues for future exploration into 's biosynthetic capabilities, potentially leading to novel antifungal compounds. Our work underscores the importance of integrating bioinformatics and genomics for fungal research, paving the way for sustainable agricultural practices by pinpointing precise molecular interventions against . This study sets a foundation for further investigations into the fungus's secondary metabolism, with implications for biotechnology and crop disease management.

摘要

利用生物信息学工具,本研究扩展了我们对 的次生代谢物的理解,鉴定了聚酮合酶 (PKS)、非核糖体肽合酶 (NRPS)、倍半萜环化酶 (STC)、二萜环化酶 (DTC) 和二甲基烯丙基色氨酸合酶 (DMATS) 家族中的新基因。这些发现丰富了与 's 致病性和生态适应性相关的遗传框架,为未知代谢途径提供了深入了解。值得注意的是,以前未注释基因的发现为开发靶向抗真菌策略提供了新的分子靶标,有望增强作物保护并推进我们对真菌生物化学的理解。这项研究不仅拓宽了已知次生代谢物的范围,还为未来探索 的生物合成能力开辟了道路,可能会产生新的抗真菌化合物。我们的工作强调了将生物信息学和基因组学整合到真菌研究中的重要性,通过针对 的精确分子干预为可持续农业实践铺平了道路。本研究为进一步研究真菌的次生代谢奠定了基础,对生物技术和作物疾病管理具有重要意义。

相似文献

本文引用的文献

1
Fungal biotechnology: From yesterday to tomorrow.真菌生物技术:从过去到未来。
Front Fungal Biol. 2023 Mar 27;4:1135263. doi: 10.3389/ffunb.2023.1135263. eCollection 2023.
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The Gene Expression Browser.基因表达浏览器。
J Fungi (Basel). 2023 Jan 6;9(1):84. doi: 10.3390/jof9010084.
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UniProt: the Universal Protein Knowledgebase in 2023.UniProt:2023 年的通用蛋白质知识库。
Nucleic Acids Res. 2023 Jan 6;51(D1):D523-D531. doi: 10.1093/nar/gkac1052.
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GenBank.GenBank
Nucleic Acids Res. 2022 Jan 7;50(D1):D161-D164. doi: 10.1093/nar/gkab1135.

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